Explantátové kultury vyšších rostlin 30

• Main Author: Krajčová, Veronika
• Other Authors: Dušková, Jiřina
• Format: Dissertation
• Language: Czech
• Published: 2008
• Online Access: http://www.nusl.cz/ntk/nusl-291029

Krajčová V.: Explant cultures of Higher plants 30. Diplom Paper, Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Botany and Ecology, 2007, p. 62 This work aimed at biotransformation of exogenous precursor of arbutin - hydroquinone with in vitro cultures of Datura meteloides D. C. ex Dunal. Into the medium was added precursor of arbutin hydroquinone in the concetrations 100 mg/ l and 200 mg/ l. The cull was operated after 24, 48, 72, 96, 120, 144, 168 hours (1 week). The cultivationes of calluses prosecuted in the light, on the liquid medium MS [10] with addition 2, 4-diclorfenoxyacetic acid (1ml/ l) on the filter - paper bridge. Positiove resultes was obtained (TLC and HPLC analysis) in produktion of arbutin in all bridging cultures in both concetrationes (100 mg/ l, 200 mg/ l). The concentration of arbutin increased with the period of cultivation by all concetrationes of hydroquinone. The biggist value arbutin by concentration of hydroquinone 100 mg/ l was obtained by period of cultivation 168 hours, it was 0,41 %. By concentration of precursor 200 mg/ l, maximum value of arbutin 1,09 % was obtained after the biggest period of cultivation 168 hours.