Biologická aktivita obsahových látek rostlin VI. Vliv alkaloidů z různých rostlinných taxonů na acetylcholinesterázu

• Main Author: Řepová, Lucie
• Other Authors: Opletal, Lubomír
• Format: Dissertation
• Language: Czech
• Published: 2007
• Online Access: http://www.nusl.cz/ntk/nusl-280143

Řepová, L.: Biological activity of plant metabolites VI. Influence of alkaloids from several taxons of plants on acetylcholinesterase. Diploma thesis, Charles University in Prague, Faculty of Pharmacy in Hradec Králové, 2007. In content of the diploma thesis it was realized screening of the alkaloids of some taxons of tribes Papaveraceae and Fumariaceae on acetylcholinesterase inhibition. It was introduced a method for obtaining optimum extracts (alkaloidal concentrates of certain type) from various morphological parts of plants Corydalis cava (L.) Schweigg. et Koerte (root), Uncaria tomentosa (WILLD.) DC. (bark), Tabebuia impetiginosa (Mart. ex DC.) Stangl. (bark), Peumus boldus (Molina) (leaf) a Papaver somniferum L. (poppy head). After the preparation of primary extract, its acidification and preliminary cleaning, extracts of different basicity were prepared by extraction either by diethyl ether (L, pH ∼2; A, pH ∼8; B, pH ∼12) or by chloroform (A1, pH~8; J, pH ∼3; E, pH ∼10). Single types of extracts were eluted by means of TLC metod both in neutral and in alkaline system of solvents on the silica gel and were tested on contents of alkaloids (detected by Dragendorff agent). Extracts contaning alkaloids were further checked together with standards (physostigmine and galanthamine) by aid of...