| Summary: | Bc. Šárka Kroupová Effects of humic substances on the experimentally injured gastrointestinal tract in rats. Diploma work Charles University in Prague, Faculty of Pharmacy in Hradec Králové Study programme: Special worker in laboratory methods Background: The aim of those diploma work was find out what effects have humic substances on experimentally injured gastrointestinal tract by cytostatic, 5- fluorouracil, in rats. Methods: The experiment proceeded five days and it was used twenty-four rats of Wistar breed. They were divided into four groups (E, F, G, H). Every group contained six individuals. The second and the fourth day animals were located into the metabolism cages. We administered sense solution of LAMA test (LAMA solution) them. LAMA test is test of intestinal permeability. It is measured concentrations of sugars (lactulosa, manitol, sucralosa, xylosa) excluded by urine for five hours. The third day was handed to 5-fluorouracil to the animals. Throughout experiment groups had access to drinkable water, just with difference, that the groups E and G had water without admixture, groups F and H had water with admixture of humic substances. The fifth day animals were euthanized by ethereal narcosis and removed them samples of tissue from small intestine. We were processed the samples and...
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