Zkoumání membránových vlastností lipozomů pomocí měření zeta potenciálu

• Main Author: Kortusová, Miroslava
• Other Authors: Miletín, Miroslav
• Format: Dissertation
• Language: English
• Published: 2009
• Online Access: http://www.nusl.cz/ntk/nusl-279505

The most comfortable drug administration is the oral one. Unfortunately the protein or peptide substances are not stable in conditions present in the digestive system. Also, thanks to their size and polaritz they are very badly absorbed through the wall of the intestine so it is necessary to modify and specially protect them. Liposomes appear to be suitable candidates - particles with phospholipid membrane not only can protect peptides and proteins against the aggressive environment in the gastrointestinal system they also improve their absorption. Liposomes could be prepared of the wide scale of different phospholipids, surface active substances or bile acid salts. The aim of this research is to prepare liposomes with addition of rarely used substances in practice and substances (particularly cholylsarcosine, stearylamine and d-α-tocopherol polyethylene glycol succinate) and find out some of their surface properties crucial for the further use in liposomes. Liposomes were prepared by film method followed by hydration and manual extrusion through a membrane with defined pore size of 200nm. The average vesicle size was defined by the method of photon - correlation spectroscopy (PCS). We succeeded in confirmation that the liposome particle size is also dependent on the constitution of the lipid...