Analýza protichřipkových léčiv s využitím elektroforetických metod
In this study, micellar elektrokinetic chromatography method (MEKC) was developed for the determination of paracetamol (PA), caffein (CO), ascorbic acid (AA) and phenylephrine (FE) in a pharmaceutical preparation. Separation was carried out in a fused silica capillary (31,2 cm x 50 m i.d. effective...
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| Format: | Dissertation |
| Language: | Czech |
| Published: |
2009
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| Online Access: | http://www.nusl.cz/ntk/nusl-279500 |
| Summary: | In this study, micellar elektrokinetic chromatography method (MEKC) was developed for the determination of paracetamol (PA), caffein (CO), ascorbic acid (AA) and phenylephrine (FE) in a pharmaceutical preparation. Separation was carried out in a fused silica capillary (31,2 cm x 50 m i.d. effective length 21 cm) at 20 kV with UV detection at 190 nm and 265 nm. Optimized background electrolyte (BGE) was 100 mM boric acid containing 100 mM sodium dodecyl sulfate (SDS) as a surfactant and 10 % (v/v) of methanol. pH* 7,8 was found to be optimal (modified by 50 mM sodium tetraborate). Methylparaben (MP) was used as internal standard. This validated method has been successfully applied for the analysis of commercially available pharmaceutical product (Coldrex tbl.). |
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