Studium radioaktivního značení hyaluronové kyseliny oxidativní jodací

Study of radiolabelling of hyaluronic acid by oxidative iodination Diploma paper Eliška Dvořáčková 2009 Charles University in Prague, Pharmaceutical Faculty in Hradec Králové Department of biophysics and physical chemistry In the frame of this work the literature describing characteristics and possi...

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Main Author: Dvořáčková, Eliška
Other Authors: Lázníčková, Alice
Format: Dissertation
Language:Czech
Published: 2009
Online Access:http://www.nusl.cz/ntk/nusl-279148
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spelling ndltd-nusl.cz-oai-invenio.nusl.cz-2791482017-06-27T04:40:30Z Studium radioaktivního značení hyaluronové kyseliny oxidativní jodací Study of radiolabelling of hyaluronic acid by oxidative iodination Dvořáčková, Eliška Lázníčková, Alice Kubíček, Vladimír Study of radiolabelling of hyaluronic acid by oxidative iodination Diploma paper Eliška Dvořáčková 2009 Charles University in Prague, Pharmaceutical Faculty in Hradec Králové Department of biophysics and physical chemistry In the frame of this work the literature describing characteristics and possible applications of hyaluronic acid and methods of radiolabelling of compounds with a view to the radioiodination was read up. In experimental part of the task two methods of oxidative iodination of hyaluronic acid modified by tyramine with the lowest degree of modification ( HA - TM - 1 ) was examined. Chloramine method, used as first one, turned out ineligible as due to presence of oxidative agent in the phase with hyaluronic acid a partial degradation of the sequence occurred. Another method of iodination with the use of iodogen is heterogenous reaction, oxidative agent is present as an insoluble deposit on the walls of the reaction vial. This method of radiolabelling was more moderate to hyaluronic acid. The reaction was interrupted by applying the reaction system on the column with Sephadex G - 50 wherby the labelled compound was separated from the aggressive reactive environment. It turned out that this way of refinement of the labelled product does not eliminate the rests of the radioactive iodide.... 2009 info:eu-repo/semantics/masterThesis http://www.nusl.cz/ntk/nusl-279148 cze info:eu-repo/semantics/restrictedAccess
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language Czech
format Dissertation
sources NDLTD
description Study of radiolabelling of hyaluronic acid by oxidative iodination Diploma paper Eliška Dvořáčková 2009 Charles University in Prague, Pharmaceutical Faculty in Hradec Králové Department of biophysics and physical chemistry In the frame of this work the literature describing characteristics and possible applications of hyaluronic acid and methods of radiolabelling of compounds with a view to the radioiodination was read up. In experimental part of the task two methods of oxidative iodination of hyaluronic acid modified by tyramine with the lowest degree of modification ( HA - TM - 1 ) was examined. Chloramine method, used as first one, turned out ineligible as due to presence of oxidative agent in the phase with hyaluronic acid a partial degradation of the sequence occurred. Another method of iodination with the use of iodogen is heterogenous reaction, oxidative agent is present as an insoluble deposit on the walls of the reaction vial. This method of radiolabelling was more moderate to hyaluronic acid. The reaction was interrupted by applying the reaction system on the column with Sephadex G - 50 wherby the labelled compound was separated from the aggressive reactive environment. It turned out that this way of refinement of the labelled product does not eliminate the rests of the radioactive iodide....
author2 Lázníčková, Alice
author_facet Lázníčková, Alice
Dvořáčková, Eliška
author Dvořáčková, Eliška
spellingShingle Dvořáčková, Eliška
Studium radioaktivního značení hyaluronové kyseliny oxidativní jodací
author_sort Dvořáčková, Eliška
title Studium radioaktivního značení hyaluronové kyseliny oxidativní jodací
title_short Studium radioaktivního značení hyaluronové kyseliny oxidativní jodací
title_full Studium radioaktivního značení hyaluronové kyseliny oxidativní jodací
title_fullStr Studium radioaktivního značení hyaluronové kyseliny oxidativní jodací
title_full_unstemmed Studium radioaktivního značení hyaluronové kyseliny oxidativní jodací
title_sort studium radioaktivního značení hyaluronové kyseliny oxidativní jodací
publishDate 2009
url http://www.nusl.cz/ntk/nusl-279148
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AT dvorackovaeliska studyofradiolabellingofhyaluronicacidbyoxidativeiodination
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