| Summary: | Novotná M.: Biological Aktive Plant Metabolites 2. Alkaloids of Chelidonium majus L. and Their Inhibitory Activity against Acetylcholinesterase. Diploma thesis, Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Botany and Ecology, Hradec Králové 2010, 64 p. The aim of diploma thesis was to process of pool of alkaloids of Chelidonium majus, chlorids insoluble in chlorophorm for at least one alkaloid isolated in pure form and for the determination of anticholinesterase, antihelminth and antioxidant activity. Of the pool in this thesis were isolated by column chromatography two alkaloids. Based on NMR and MS study was the first substance MN-1 identified as protopine. The second substance MN-2 was also subjected to MS and NMR structural studies and identified as allokryptopine. Both alkaloids were previously isolated from Chelidonium majus. The both isolated substances were subjected to studies on their inhibitory activity against human erythrocyte acetylcholinesterase and human serum butyrylcholinesterase. Values were determined (protopine: IC50 (AChE) = 423 ± 12,7 µM, IC50 (BuChE) = 322 ± 9,6 µM, allokryptopine: IC50 (AChE) = 250 ± 7,5 µM, IC50 (BuChE) = 530 ± 15,9 µM). Both alkaloids showed low activity in comparison. With standard alkaloid...
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