Změny proteinové exprese vyvolané kyselinou valproovou u lidské T-lymfocytární linie MOLT-4

• Main Author: Tichá, Zuzana
• Other Authors: Kvasničková, Eva
• Format: Dissertation
• Language: Czech
• Published: 2009
• Online Access: http://www.nusl.cz/ntk/nusl-279123

Univerzita Karlova Farmaceutická fakulta Katedra biochemických věd EFFECT OF VALPROIC ACID (VA) ON CHANGES IN EXPRESSION OF PROTEINS OF HUMAN T-LYMPHOCYTE LEUKEMIA CELL LINE MOLT-4 Valproic acid (VA) is histondeacetylase inhibitor (HDACI) and it leads to inhibition in proliferation and apoptosis induction during incubation with leukemia cells. The aim of our article was to determine the changes in expression of proteins after incubation leukemia cells with VA. After 4-hours-long incubation of MOLT-4 cells with 2 and 4 mmol/l VA, the nuclear extracts from MOLT-4 cells were used to detection of changes in levels of proteins. The nuclear extracts were separated for first dimension-IEF in immobilized pH 4-7 gradient. Immediately after being focused, the proteins were separated for electophoretic way in the second dimension, 9-16% gradient DS was used. Proteins separated by 2D electrophoresis were visualized by silver staining , and silver-stained gels were digitized using a laser personal densitometer. Protein maps were quantified using a Melanie 3 software package. Proteins were identified by MALDI mass spectrometer. The finding protein process was finished by using ProFound and PeptIdent programs. Identification process of protein spots exhibiting significant abundance alterations induced by VA help to...