Diagnostika invazivního meningokokového onemocnění metodou PCR

• Main Author: Bronská, Eva
• Other Authors: Marešová, Vilma
• Format: Doctoral Thesis
• Language: Czech
• Published: 2007
• Online Access: http://www.nusl.cz/ntk/nusl-271579

lntroduction: lnvasive meningococcal disease continues to be a life-threatening condition. Rapid diagnosis is important for the administration of appropriate treatment. Our study focuses on evaluation of importance of PCR method for diagnosis of meningococcal disease. We describe in detail the dynamics of PCR method and its relationship to other diagnostic methods (LA, culture) and to the course of the disease. We also evaluated the influence of previously appl ied antibiotics therapy to results ofthese diagnostic methods. Methods: We have investigated cerebrospinal fluid and serum samples collected during the 1 sl week of hospitalization from patients hospitalized at the department of infectious diseases with laboratory confirmed invasi ve meningococcal disease. Bacterial DNA was isolated from biological material by Qiagen kit and one-step and seminested PCR method was used. PCR products were detected on the 2% gel electrophoresis. In parallel, we performed culture and LA methods. Results: The PCR positivity in cerebrospinal fluid and serum achieved 92 % and 46 %, respectively, LA positivity in cerebrospinal fluid, serum and urine achieved 47 %, 42 % and 24 %, respectively. Culture of CSF and blood was positive only in 35 % and 39 %, respectively. The latest PCR positivity was detected at Day 7 in...