Příprava rekombinantních proteinů pro použití v tkáňových kulturách
cDNA derived from exons 2 and 3 of the Ser2 (Sericin 2) gene of Bombyx mori and encoding 121 amino acids was expressed in Escherichia coli as a fusion protein with hexahistidine to allow purification by affinity chromatogramy on Ni-resine column. Several expression systems were used and the codon us...
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| Format: | Dissertation |
| Language: | Czech |
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2012
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| Online Access: | http://www.nusl.cz/ntk/nusl-135818 |