The use of whole blood cell cultures as a model for assessing the effects of SeptilinTM on the immune system.

• Main Author: Hoosen, Mujeeb
• Other Authors: Pool, E.J.
• Language: en
• Published: University of the Western Cape 2018
• Subjects: Artemisia afra; Cell mediated immunity; Cytokines; Herbal medicinal products; Human whole blood culture; Humoral immunity; Inflammation; RAW 264.7 macrophages; Aspalathus linearis (rooibos); SeptilinTM
• Online Access: http://hdl.handle.net/11394/6187

Magister Scientiae - MSc (Medical BioSciences) === In the past three decades there has been a huge increase in the use of herbal medicine globally. The active principles of these herbal medicines are mostly unknown with supportive evidence for safety and efficacy very rare. SeptilinTM is a phytopharmaceutical formulation which is recommended for the treatment and management of various infections. It has been claimed to have immunomodulatory actions that potentiates the body's immune response. The immunomodulatory activity of SeptilinTM has not been well investigated via appropriate in vitro models. Therefore this study was undertaken to investigate the in vitro effects of SeptilinTM on biomarkers of specific immune pathways by using WBC. Stimulated and unstimulated WBC were incubated with the product. Enzyme linked immunosorbent assays were used to screen for IL-6, IL-10, and IFN? as biomarkers for inflammation, humoral immunity, and cell mediated immunity, respectively. Results show that the presence of SeptilinTM in LPS stimulated WBC has no effect on the release of IL-6 and IFN? production but stimulated IL-10 production. SeptilinTM in unstimulated WBC has no effect on the release of IL-10 and IFN? production but stimulatory effects on IL-6 production.