Genetic analysis of the Cape Sand Frog, Tomopterna delalandii (Tschudi 1838)

Magister Scientiae (Biodiversity and Conservation Biology) - MSc (Biodiv and Cons Biol) === Tomopterna delalandii occurs throughout the west coast, Western Cape and south coast of South Africa. This range stretches across three distinct biogeographical assemblages. Based on historical records and th...

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Bibliographic Details
Main Author: Wilson, Lyle
Other Authors: Channing, Alan
Language:en
Published: University of the Western Cape 2016
Subjects:
Online Access:http://hdl.handle.net/11394/4866
Description
Summary:Magister Scientiae (Biodiversity and Conservation Biology) - MSc (Biodiv and Cons Biol) === Tomopterna delalandii occurs throughout the west coast, Western Cape and south coast of South Africa. This range stretches across three distinct biogeographical assemblages. Based on historical records and the fact that Tomopterna is a genus of cryptic frogs, it is possible that there are unknown genetic variations within the species. To investigate whether population structure is present within T. delalandii a mitochondrial gene and nuclear gene, 16S and Tyrosinase, was sequenced from across the range. Haplotype networks and cladograms were constructed to look at the relationship between the genetic samples. This revealed definite population structuring between samples from the western edge of the range and samples from the eastern edge of the range. It also revealed that samples taken from the northern edge of the range, while matching general T. delalandii tadpole morphology, are genetically different. Further study needs to be made into the identity of this new form. This study shows that by using both mitochondrial and nuclear DNA patterns can be discovered about the genetic structure of a species as well as revealing a new one.