Using sodium bisulphite treatment and PCR to construct mammalian anti-HIV-1 long hairpin RNA expression cassettes

M.Tech. === RNA interference (RNAi) is a gene silencing mechanism that uses short RNA duplexes to block gene expression. This mechanism has been widely explored to determine functions of genes. Furthermore, this phenomenon has been used to silence unwanted genes such as viral genes. RNAi has been su...

Full description

Bibliographic Details
Main Author: Lugongolo, Masixole Yvonne
Published: 2012
Subjects:
Online Access:http://hdl.handle.net/10210/4686