Expression of HPV-16 L2 in plants

• Main Author: Pereira, Roman
• Other Authors: Rybicki, Ed
• Format: Dissertation
• Language: English
• Published: University of Cape Town 2014
• Subjects: Cell Biology
• Online Access: http://hdl.handle.net/11427/4317

Includes bibliographical references (leaves 77-84). === This study demonstrates high level expression of L2, 25 mg.kg⁻¹of leaf material, is achievable. Interestingly, expression was best when coded for by a mammalian codon-optimized form of the L2 gene as opposed to the wildtype or plant codon-optimized (plantized) genes. Moreover, real time PCR revealed limited levels of transcript when coded for by the plantized gene in comparison to the other genes. A set of vectors which target the protein to the cytoplasm, the endoplasmic reticulum (ER), chloroplast or apoplastic space were Expression of HPV -16 L2 in Plants used and it was found that targeting of the protein had no effect on its expression levels.