Optimisation of the mycobacterial replicon of an E. coli-mycobacterial shuttle vector

Includes bibliographical references (leaves 89-95). === This study aimed to investigate whether the mycobacterial replicon may also play a role. The common mycobacterial replicon in episomal E. coli-mycobacterial shuttle vectors contains the truncated rap gene, encoding an auxiliary replication fact...

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Main Author:	Griffin, Sarah
Other Authors:	Chapman, Rosamund
Format:	Dissertation
Language:	English
Published:	University of Cape Town 2014
Subjects:	Medical Virology
Online Access:	http://hdl.handle.net/11427/2727

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spelling	ndltd-netd.ac.za-oai-union.ndltd.org-uct-oai-localhost-11427-27272020-10-06T05:10:48Z Optimisation of the mycobacterial replicon of an E. coli-mycobacterial shuttle vector Griffin, Sarah Chapman, Rosamund Medical Virology Includes bibliographical references (leaves 89-95). This study aimed to investigate whether the mycobacterial replicon may also play a role. The common mycobacterial replicon in episomal E. coli-mycobacterial shuttle vectors contains the truncated rap gene, encoding an auxiliary replication factor, and the repA and repB genes which code for essential replication proteins. Specific aspects of this typical mycobacterial replicon were identified as potential targets to improve stability and increase recombinant protein expression levels, and were modified accordingly. 2014-07-28T08:19:16Z 2014-07-28T08:19:16Z 2007 Master Thesis Masters MSc http://hdl.handle.net/11427/2727 eng application/pdf University of Cape Town Faculty of Health Sciences Division of Virology
collection	NDLTD
language	English
format	Dissertation
sources	NDLTD
topic	Medical Virology
spellingShingle	Medical Virology Griffin, Sarah Optimisation of the mycobacterial replicon of an E. coli-mycobacterial shuttle vector
description	Includes bibliographical references (leaves 89-95). === This study aimed to investigate whether the mycobacterial replicon may also play a role. The common mycobacterial replicon in episomal E. coli-mycobacterial shuttle vectors contains the truncated rap gene, encoding an auxiliary replication factor, and the repA and repB genes which code for essential replication proteins. Specific aspects of this typical mycobacterial replicon were identified as potential targets to improve stability and increase recombinant protein expression levels, and were modified accordingly.
author2	Chapman, Rosamund
author_facet	Chapman, Rosamund Griffin, Sarah
author	Griffin, Sarah
author_sort	Griffin, Sarah
title	Optimisation of the mycobacterial replicon of an E. coli-mycobacterial shuttle vector
title_short	Optimisation of the mycobacterial replicon of an E. coli-mycobacterial shuttle vector
title_full	Optimisation of the mycobacterial replicon of an E. coli-mycobacterial shuttle vector
title_fullStr	Optimisation of the mycobacterial replicon of an E. coli-mycobacterial shuttle vector
title_full_unstemmed	Optimisation of the mycobacterial replicon of an E. coli-mycobacterial shuttle vector
title_sort	optimisation of the mycobacterial replicon of an e. coli-mycobacterial shuttle vector
publisher	University of Cape Town
publishDate	2014
url	http://hdl.handle.net/11427/2727
work_keys_str_mv	AT griffinsarah optimisationofthemycobacterialrepliconofanecolimycobacterialshuttlevector
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Optimisation of the mycobacterial replicon of an E. coli-mycobacterial shuttle vector

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