A stability-indicating liquid chromatographic method for the analysis of erythromycin in stored biological fluids using amperometric detection

A stability-indicating liquid chromatographic method for the analysis of erythromycin in stored biological fluids using amperometric detection

A simple, sensitive and reliable high-performance liquid chromatographic procedure has been developed for the determination of erythromycin in human serum and urine using amperometric detection. A solid-phase extraction procedure was used followed by chromatography on a reverse-phase column. The mea...

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Main Authors: Stubbs, C, Haigh, J M, Kanfer, I
Format: Others
Language:English
Published: 1987
Online Access:http://hdl.handle.net/10962/d1006592
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spelling ndltd-netd.ac.za-oai-union.ndltd.org-rhodes-vital-64302018-03-06T03:55:31ZA stability-indicating liquid chromatographic method for the analysis of erythromycin in stored biological fluids using amperometric detectionStubbs, CHaigh, J MKanfer, IA simple, sensitive and reliable high-performance liquid chromatographic procedure has been developed for the determination of erythromycin in human serum and urine using amperometric detection. A solid-phase extraction procedure was used followed by chromatography on a reverse-phase column. The mean recovery of erythromycin from serum and urine was 80%. This method allows both erythromycin and its principle degradation product, anhydroeythromycin, to be determined during a period of sample storage at 4 degree C and minus 15 degree C. The method is sufficiently sensitive and precise and is thus highly suited for use in both pharmacokinetic and stability studies.1987Article11 pagespdfvital:6430http://hdl.handle.net/10962/d1006592English
collection NDLTD
language English
format Others
sources NDLTD
description A simple, sensitive and reliable high-performance liquid chromatographic procedure has been developed for the determination of erythromycin in human serum and urine using amperometric detection. A solid-phase extraction procedure was used followed by chromatography on a reverse-phase column. The mean recovery of erythromycin from serum and urine was 80%. This method allows both erythromycin and its principle degradation product, anhydroeythromycin, to be determined during a period of sample storage at 4 degree C and minus 15 degree C. The method is sufficiently sensitive and precise and is thus highly suited for use in both pharmacokinetic and stability studies.
author Stubbs, C
Haigh, J M
Kanfer, I
spellingShingle Stubbs, C
Haigh, J M
Kanfer, I
A stability-indicating liquid chromatographic method for the analysis of erythromycin in stored biological fluids using amperometric detection
author_facet Stubbs, C
Haigh, J M
Kanfer, I
author_sort Stubbs, C
title A stability-indicating liquid chromatographic method for the analysis of erythromycin in stored biological fluids using amperometric detection
title_short A stability-indicating liquid chromatographic method for the analysis of erythromycin in stored biological fluids using amperometric detection
title_full A stability-indicating liquid chromatographic method for the analysis of erythromycin in stored biological fluids using amperometric detection
title_fullStr A stability-indicating liquid chromatographic method for the analysis of erythromycin in stored biological fluids using amperometric detection
title_full_unstemmed A stability-indicating liquid chromatographic method for the analysis of erythromycin in stored biological fluids using amperometric detection
title_sort stability-indicating liquid chromatographic method for the analysis of erythromycin in stored biological fluids using amperometric detection
publishDate 1987
url http://hdl.handle.net/10962/d1006592
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