Propagation and stress physiology of selected Subtropical Thicket species : towards increasing biodiversity at rehabilitation sites

• Main Author: Louw, Merika
• Format: Others
• Language: English
• Published: Nelson Mandela Metropolitan University 2012
• Subjects: Biodiversity conservation; Plant physiology; Stress (Physiology); Plant propagation
• Online Access: http://hdl.handle.net/10948/d1021115

Sundays Thicket and Spekboomveld vegetation in the Eastern Cape have experienced intensive and extensive degradation due to over-browsing by domestic stock. The Subtropical Thicket Restoration Project aims to take advantage of the high carbon sequestration potential of Portulacaria afra (Spekboom) and other Thicket species, to rehabilitate degraded Thicket by restoring biodiversity and ecosystem services, create job opportunities and socio-economic upliftment in local communities, and promote the trade of carbon credits generated by planting and „farming‟ with Spekboom truncheons, rather than domestic stock. Plant material (seeds and length of stem) was sourced from the farm Krompoort, outside Uitenhage in the Eastern Cape. The effectiveness of four different rooting media i.e. plain pool filter sand and 1:1 mixtures of pool filter sand with perlite, potting soil and Thicket soil, on the rooting of ten Thicket species, were tested. Species with the greatest percentage strike and mean root length on stem cuttings were succulent species, Crassula ovata (84 percent strike) and Portulacaria afra (97 percent), as was expected. The application of rooting hormone Seradix© No. 3 did not significantly promote cutting strike or increase mean root length in C. ovata and P. afra. Woody canopy shrub species with the greatest cutting strike and mean root length, overall, were Rhigozum obovatum (24 percent), Lycium cinereum and L. oxycarpum (21 percent), and Searsia longispina (19 percent). These species, as well as Grewia robusta (4 percent), are considered „easy-to-root‟ species, or of sufficient functional value in terms of their spinescence, flower and fruit production, and soil-binding capabilities. Very low percentage strike (<1 percent) and mean root length were achieved in Azima tetracantha, Carissa bispinosa and Gymnosporia polyacantha subsp. polyacantha. These species are considered unsuitable for propagation for rehabilitation purposes. Crassula ovata, L. cinereum, L. oxycarpum, P. afra and S. longispina cuttings produced longer roots when planted in Thicket soil, the same having been found in R. obovatum cuttings planted in perlite. Thicket soil was, therefore, best at promoting cutting strike and root growth in „easy-to-root‟ species. Plain pool filter sand was the only medium in which A. tetracantha, C. bispinosa and G. robusta, cuttings rooted, and perlite the only medium in which G. polyacantha subsp. polyacantha cuttings rooted. Potting soil did not promote significant cutting strike or root growth in any of the species tested. Physiological variables i.e. photosynthetic efficiency (chlorophyll a fluorescence, Fv/Fm) and stomatal conductance (mmol H2O m-2 s-1) were measured for ten Thicket species, including Portulacaria afra. Control plants were watered well once a week, and treatment plants were dried out for 30 days, rewatered on the 30th day and their recovery from drought stress monitored for a further 17 days. Species that responded poorly to drought stress were Gymnosporia buxifolia and Putterlickia pyracantha. In addition to Portulacaria afra, species that showed the fastest recovery and resprout after rewatering, were: Crassula ovata, Ehretia rigida, Grewia robusta, Lycium ferocissimum, Rhigozum obovatum and Searsia longispina. These species produced the smallest decline in volumetric moisture content of soil, and had the lowest decline in photosynthetic efficiency and stomatal conductance during simulated drought. Unlike C. ovata and P. afra, which are CAM or C3-CAM switching species, mortality of transplants will most likely be high, if not total, during transplantation, as this study was done at lower light and temperature, and higher humidity levels than experienced at rehabilitation sites. This study has shown that the reintroduction of propagated woody canopy shrubs and trees into degraded Thicket sites does not appear to be a practical or economical method of actively restoring biodiversity to rehabilitation sites. As woody climax species have been shown to return to sites planted with Spekboom truncheons through „natural regeneration‟ within approximately 50 years, future research efforts should focus on optimising restoration site selection and planting techniques in order to maximize carbon sequestration potential of planted truncheons, which will, in the long term, result in an environment that can support regeneration of the biodiversity to something resembling intact Thicket.