A study to evaluate variable number of tandem repeat DNA polymorphisms in disputed paternity testing

• Main Author: Schlaphoff, Theresa Elizabeth-Anne
• Other Authors: Truter, E. J.
• Language: en
• Published: Cape Technikon 2016
• Online Access: http://hdl.handle.net/20.500.11838/2250

Thesis (MTech (Medical Technology))--Cape Technikon, 1993. === The use of genetic marker testing to resolve cases of disputed paternity, is well established. The number and range of systems used depends on the expertise of the laboratory, and for this reason various laboratories offer different systems. Standard testing includes tests in the following genetic marker systems: human leukocyte antigen (tissue) typing; red cell blood groups; and red cell enzyme and serum protein testing. The Provincial Laboratory for Tissue Immunology currently offers a range of 16 genetic marker systems capable of excluding >99% of falsely accused men. Following the discovery DNA polymorphisms, particularly VNTR DNA polymorphisms, and the commercial availability of VNTR DNA probes, PLTI decided to offer this service to our clients. This study was the initial phase in the establishment of a VNTR DNA typing laboratory and covered the determination of inter-and intra-gel accuracy and precision, selection of restriction enzyme/probe combination, and evaluation and comparison of the results of 100 disputed paternity cases tested using both standard and VNTR DNA typing.