Label-Free CD8+ T-cell Purification and Electroporation in Relation to CAR T-cell Therapy

Label-Free CD8+ T-cell Purification and Electroporation in Relation to CAR T-cell Therapy

Immunotherapy is becoming recognized as a superior treatment for cancer. In recent years, chimeric antigen receptor (CAR) therapy is among the immunotherapies that has had growing success rates. CAR T-cell therapy takes patient’s T-cells and encodes them with a CAR expressing gene, which can then ta...

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Bibliographic Details
Main Author: Ringwelski, Beth Anne
Format: Others
Published: North Dakota State University 2021
Subjects:
cancer
dielectrophoresis
microfluidics
Online Access:https://hdl.handle.net/10365/31881
id ndltd-ndsu.edu-oai-library.ndsu.edu-10365-31881
record_format oai_dc
spelling ndltd-ndsu.edu-oai-library.ndsu.edu-10365-318812021-09-28T17:11:31Z Label-Free CD8+ T-cell Purification and Electroporation in Relation to CAR T-cell Therapy Ringwelski, Beth Anne cancer dielectrophoresis microfluidics Immunotherapy is becoming recognized as a superior treatment for cancer. In recent years, chimeric antigen receptor (CAR) therapy is among the immunotherapies that has had growing success rates. CAR T-cell therapy takes patient’s T-cells and encodes them with a CAR expressing gene, which can then target their cancer cells. However, there are some dangers associated with this therapy. If a cancer cell is mistakenly transfected with the CAR molecule, it can become resistant to the therapy. Using the electric properties of the cells, we have created a technique that can purify the T-cells from the remaining cancer cells using microfluidics and dielectrophoresis (DEP). Then, to further improve the therapy, the sample is electroporated following being patterned using DEP forces, which transfects the cells without using viral vectors and provides longer CD19 expression. 2021-05-13T19:13:34Z 2021-05-13T19:13:34Z 2020 text/thesis https://hdl.handle.net/10365/31881 NDSU policy 190.6.2 https://www.ndsu.edu/fileadmin/policy/190.pdf application/pdf North Dakota State University
collection NDLTD
format Others
sources NDLTD
topic cancer
dielectrophoresis
microfluidics
spellingShingle cancer
dielectrophoresis
microfluidics
Ringwelski, Beth Anne
Label-Free CD8+ T-cell Purification and Electroporation in Relation to CAR T-cell Therapy
description Immunotherapy is becoming recognized as a superior treatment for cancer. In recent years, chimeric antigen receptor (CAR) therapy is among the immunotherapies that has had growing success rates. CAR T-cell therapy takes patient’s T-cells and encodes them with a CAR expressing gene, which can then target their cancer cells. However, there are some dangers associated with this therapy. If a cancer cell is mistakenly transfected with the CAR molecule, it can become resistant to the therapy. Using the electric properties of the cells, we have created a technique that can purify the T-cells from the remaining cancer cells using microfluidics and dielectrophoresis (DEP). Then, to further improve the therapy, the sample is electroporated following being patterned using DEP forces, which transfects the cells without using viral vectors and provides longer CD19 expression.
author Ringwelski, Beth Anne
author_facet Ringwelski, Beth Anne
author_sort Ringwelski, Beth Anne
title Label-Free CD8+ T-cell Purification and Electroporation in Relation to CAR T-cell Therapy
title_short Label-Free CD8+ T-cell Purification and Electroporation in Relation to CAR T-cell Therapy
title_full Label-Free CD8+ T-cell Purification and Electroporation in Relation to CAR T-cell Therapy
title_fullStr Label-Free CD8+ T-cell Purification and Electroporation in Relation to CAR T-cell Therapy
title_full_unstemmed Label-Free CD8+ T-cell Purification and Electroporation in Relation to CAR T-cell Therapy
title_sort label-free cd8+ t-cell purification and electroporation in relation to car t-cell therapy
publisher North Dakota State University
publishDate 2021
url https://hdl.handle.net/10365/31881
work_keys_str_mv AT ringwelskibethanne labelfreecd8tcellpurificationandelectroporationinrelationtocartcelltherapy
_version_ 1719485800715911168

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