A Far-Field Radio Frequency Experimental Exposure System and 31 Day Exposure Experiment on Understanding Mice

• Main Author: Hansen, Jared William
• Format: Others
• Published: North Dakota State University 2018
• Online Access: https://hdl.handle.net/10365/27883

Many studies have been performed on exploring the effects of radio frequency energy on biological function in vivo. In particular, gene expression results have been inconclusive due, in part, to a lack of a standardized experimental procedure. This thesis describes a new far field RF exposure system for unrestrained murine models that reduces experimental error and a 31 day experiment using mice in vivo. The experiment uses whole body exposure to continuous RF energy at 2.45 GHz on unrestrained, in vivo mice. Using RNA-seq to analyze the entire murine genome, the data is statistically analyzed using combinations and empirical p-values. The analyzed data’s genome are explored using Ingenuity Pathway Analysis to locate gene functional groups within heart tissues. Results show an intriguing finding of a discrete/continuous system due to radio frequency energy, along with genes alteration found in heart functional groups. === NCI Cancer Center Support Grant #P30 CA91842 to the Siteman Cancer Center and by ICTS/CTSA Grant# UL1TR000448 from the National Center for Research Resources (NCRR) === ND Venture Grant 14-08-J1-66