Marker Assisted Selection Increases the Efficiency of Breeding for Potato Virus Y Resistance in Potato

Potato Virus Y (PVY) is an important virus of potato due to the non-persistent mode of transmission by aphids causing yield losses. Genetic resistance is the recommended control since insecticides cannot adequately control the spread of PVY by aphids. The gene Ryadg from S. tuberosum ssp. andigena p...

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Main Author: Harchenko, Whitney Ann
Format: Others
Published: North Dakota State University 2018
Subjects:
Online Access:https://hdl.handle.net/10365/27539
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spelling ndltd-ndsu.edu-oai-library.ndsu.edu-10365-275392021-09-28T17:10:58Z Marker Assisted Selection Increases the Efficiency of Breeding for Potato Virus Y Resistance in Potato Harchenko, Whitney Ann Botany. Potato Virus Y (PVY) is an important virus of potato due to the non-persistent mode of transmission by aphids causing yield losses. Genetic resistance is the recommended control since insecticides cannot adequately control the spread of PVY by aphids. The gene Ryadg from S. tuberosum ssp. andigena provides resistance to all strains of PVY. This gene has genetically been mapped to chromosome XI, and linked polymerase chain reaction (PCR) based DNA markers have been identified. This study identified PVY resistant progeny by the use of the molecular sequence-characterized amplified region (SCAR) marker RYSC3. The RYSC3 marker allowed a simple and fast approach to determine if the Ryadg gene was present in the seedling family populations evaluated. The RYSC3 marker identified 16 families with progeny segregating for the Ryadg gene. Progeny segregated 1:1 for PVY resistance, fitting the model simplex (Ryryryry) for the Ryadg allele. 2018-02-13T16:53:21Z 2018-02-13T16:53:21Z 2014 text/thesis https://hdl.handle.net/10365/27539 NDSU policy 190.6.2 https://www.ndsu.edu/fileadmin/policy/190.pdf application/pdf North Dakota State University
collection NDLTD
format Others
sources NDLTD
topic Botany.
spellingShingle Botany.
Harchenko, Whitney Ann
Marker Assisted Selection Increases the Efficiency of Breeding for Potato Virus Y Resistance in Potato
description Potato Virus Y (PVY) is an important virus of potato due to the non-persistent mode of transmission by aphids causing yield losses. Genetic resistance is the recommended control since insecticides cannot adequately control the spread of PVY by aphids. The gene Ryadg from S. tuberosum ssp. andigena provides resistance to all strains of PVY. This gene has genetically been mapped to chromosome XI, and linked polymerase chain reaction (PCR) based DNA markers have been identified. This study identified PVY resistant progeny by the use of the molecular sequence-characterized amplified region (SCAR) marker RYSC3. The RYSC3 marker allowed a simple and fast approach to determine if the Ryadg gene was present in the seedling family populations evaluated. The RYSC3 marker identified 16 families with progeny segregating for the Ryadg gene. Progeny segregated 1:1 for PVY resistance, fitting the model simplex (Ryryryry) for the Ryadg allele.
author Harchenko, Whitney Ann
author_facet Harchenko, Whitney Ann
author_sort Harchenko, Whitney Ann
title Marker Assisted Selection Increases the Efficiency of Breeding for Potato Virus Y Resistance in Potato
title_short Marker Assisted Selection Increases the Efficiency of Breeding for Potato Virus Y Resistance in Potato
title_full Marker Assisted Selection Increases the Efficiency of Breeding for Potato Virus Y Resistance in Potato
title_fullStr Marker Assisted Selection Increases the Efficiency of Breeding for Potato Virus Y Resistance in Potato
title_full_unstemmed Marker Assisted Selection Increases the Efficiency of Breeding for Potato Virus Y Resistance in Potato
title_sort marker assisted selection increases the efficiency of breeding for potato virus y resistance in potato
publisher North Dakota State University
publishDate 2018
url https://hdl.handle.net/10365/27539
work_keys_str_mv AT harchenkowhitneyann markerassistedselectionincreasestheefficiencyofbreedingforpotatovirusyresistanceinpotato
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