Cytotoxicity and drug potentiating activity of phenylheptatriyne

• Main Author: Gray, Rachel Arrasmith
• Format: Others
• Published: FIU Digital Commons 2004
• Subjects: Biology; Life Sciences
• Online Access: http://digitalcommons.fiu.edu/etd/2433; http://digitalcommons.fiu.edu/cgi/viewcontent.cgi?article=3704&context=etd

The purpose of this study was to determine the toxicity of the phototoxin, phenylheptatriyne (PHT) to acute lymphoblastic leukemia cells (ALL) under attenuated light conditions and when exposed to ultraviolet-A light (UVA). The potential of PHT to increase sensitivity of ALL cells to the anti-cancer drug doxorubicin hydrochloride also was evaluated. An in vitro multi-drug resistance model was used consisting of the parental cell line CCRF-CEM and its p-glycoprotein (pgp-170) expressing variant CEM/VLB100. Cytotoxicity was measured using the tetrazolium bromide (MTT) reduction assay and the annexin-V-FITC / propidium iodide (PI), flow cytometric assay. The results indicate that PHT is more toxic, when not photoexcited, to the CEM/VLB100 cell line (P = 0.006). There was a significant interaction between UVA dose and PHT concentration (P < 0.001). Co-incubation of CEM/VLB100 cells with less than μM doxorubicin and 60 μM PHT, significantly decreased viability relative to doxorubicin alone (P = 0.007).