Establishment of a PCR and restriction enzyme assay for the detection of the hemochromatosis gene in normal subjects and diabetic patients

Establishment of a PCR and restriction enzyme assay for the detection of the hemochromatosis gene in normal subjects and diabetic patients

The purpose of this study was to establish a polymerase chain reaction (PCR)-restriction enzyme assay for detecting the hereditary hemochromatosis (HHC) mutation, C282Y, in gestational and gestational diabetic subjects in South Florida. DNA samples from 43 gestational subjects were amplified by PCR,...

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Main Author: Cheat Eng, Lim
Format: Others
Published: FIU Digital Commons 1998
Subjects:
Medicine and Health Sciences
Online Access:http://digitalcommons.fiu.edu/etd/2121
id ndltd-fiu.edu-oai-digitalcommons.fiu.edu-etd-3375
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spelling ndltd-fiu.edu-oai-digitalcommons.fiu.edu-etd-33752018-01-05T15:32:19Z Establishment of a PCR and restriction enzyme assay for the detection of the hemochromatosis gene in normal subjects and diabetic patients Cheat Eng, Lim The purpose of this study was to establish a polymerase chain reaction (PCR)-restriction enzyme assay for detecting the hereditary hemochromatosis (HHC) mutation, C282Y, in gestational and gestational diabetic subjects in South Florida. DNA samples from 43 gestational subjects were amplified by PCR, digested with RsaI, and analyzed by electrophoresis. An allelic frequency of 2.33%, or 4.65% heterozygosity, was observed. The assay is successful and applicable to future studies on HHC and gestational diabetes. 1998-11-18T08:00:00Z text application/pdf http://digitalcommons.fiu.edu/etd/2121 FIU Electronic Theses and Dissertations FIU Digital Commons Medicine and Health Sciences
collection NDLTD
format Others
sources NDLTD
topic Medicine and Health Sciences
spellingShingle Medicine and Health Sciences
Cheat Eng, Lim
Establishment of a PCR and restriction enzyme assay for the detection of the hemochromatosis gene in normal subjects and diabetic patients
description The purpose of this study was to establish a polymerase chain reaction (PCR)-restriction enzyme assay for detecting the hereditary hemochromatosis (HHC) mutation, C282Y, in gestational and gestational diabetic subjects in South Florida. DNA samples from 43 gestational subjects were amplified by PCR, digested with RsaI, and analyzed by electrophoresis. An allelic frequency of 2.33%, or 4.65% heterozygosity, was observed. The assay is successful and applicable to future studies on HHC and gestational diabetes.
author Cheat Eng, Lim
author_facet Cheat Eng, Lim
author_sort Cheat Eng, Lim
title Establishment of a PCR and restriction enzyme assay for the detection of the hemochromatosis gene in normal subjects and diabetic patients
title_short Establishment of a PCR and restriction enzyme assay for the detection of the hemochromatosis gene in normal subjects and diabetic patients
title_full Establishment of a PCR and restriction enzyme assay for the detection of the hemochromatosis gene in normal subjects and diabetic patients
title_fullStr Establishment of a PCR and restriction enzyme assay for the detection of the hemochromatosis gene in normal subjects and diabetic patients
title_full_unstemmed Establishment of a PCR and restriction enzyme assay for the detection of the hemochromatosis gene in normal subjects and diabetic patients
title_sort establishment of a pcr and restriction enzyme assay for the detection of the hemochromatosis gene in normal subjects and diabetic patients
publisher FIU Digital Commons
publishDate 1998
url http://digitalcommons.fiu.edu/etd/2121
work_keys_str_mv AT cheatenglim establishmentofapcrandrestrictionenzymeassayforthedetectionofthehemochromatosisgeneinnormalsubjectsanddiabeticpatients
_version_ 1718581551758835712

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