An Analysis of Efficiency and Melt Curve Effects on Quantitative Polymerase Chain Reaction (qPCR) Inhibition
Real-time or quantitative PCR (qPCR) is an innovative method used to determine the amount of amplifiable DNA in a biological sample. Typically, a fluorescent dye is introduced during thermal cycling, causing a change in fluorescent output as the double-stranded DNA (dsDNA) product accumulates. Both...
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| Format: | Others |
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FIU Digital Commons
2010
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| Online Access: | http://digitalcommons.fiu.edu/etd/329 http://digitalcommons.fiu.edu/cgi/viewcontent.cgi?article=1408&context=etd |