A comprehensive study of mammalian SNAG transcription family members

• Other Authors: Chiang, Cindy Chung-Yue.
• Format: Others
• Language: English
• Published: Florida Atlantic University
• Subjects: Cellular signal transduction; Zinc-finger proteins > Synthesis; Metalloproteins > Synthesis; Genetic transcription > Regulation
• Online Access: http://purl.flvc.org/fcla/dt/3342041

Transcriptional regulation by the family of SNAG (Snail/Gfi-1) zinc fingers has been shown to play a role in various developmental states and diseases. These transcriptional repressors have function in both DNA- and protein-binding, allowing for multiple interactions by a single family member. This work aims to characterize the SNAG members Slug, Smuc, Snail, Scratch, Gfi-1, Gfi-1B, and IA-1 in terms of both DNA-protein and protein-protein interactions. The specific DNA sequences to which the zinc finger regions bind were determined for each member, and a general consensus of TGCACCTGTCCGA, was developed for four of the members. Via these studies, we also reveal thebinding affinities of E-box (CANNTG) sequences to the members, since this core is found for multiple members' binding sites. Additionally, protein-protein interactions of SNAG members to other biological molecules were investigated. The Slug domain and Scratch domain have unknown function, yet through yeast two-hybrid screening, we were able to determine protein interaction partners for them as well as for other full length SNAG members. These protein-interacting partners have suggested function as corepressors during transcriptional repression. The comprehensive information determined from these studies allow for a better understanding of the functional relationship between SNAG-ZFPs and other genes. The collected data not only creates a new profile for each member investigated, but it also allows for further studies to be initiated from the results. === by Cindy Chiang. === Thesis (Ph.D.)--Florida Atlantic University, 2012. === Includes bibliography. === Electronic reproduction. Boca Raton, Fla., 2012. Mode of access: World Wide Web.