Molecular investigation of chemical-assisted protein rescue in ocular protein folding diseases.
In the study of alphaA-crystallin (CRYAA), G98R CRYAA was cloned into a mammalian expression vector pcDNA6-His/myc version B and the sequence was confirmed by direct sequencing. Following lipophilic transfection to lens epithelial B3 cells, the recombinant mutated CRYAA protein was highly insoluble...
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| Format: | Others |
| Language: | English Chinese |
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2010
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| Online Access: | http://library.cuhk.edu.hk/record=b6075053 http://repository.lib.cuhk.edu.hk/en/item/cuhk-344686 |