Subcellular localization and targeting mechanisms of arabidopsis endomembrane protein 12 (EMP12).
在酵母和动物细胞中,内膜蛋白(EMP)隶属于进化上保守的九跨膜结构域(TMD)蛋白家族,此类蛋白的共同结构特征是有一个很长的N 末端,紧接着九个跨膜结构域后面连着暴露于胞质的C 末端短肽。在黏菌以及酵母中,EMP 蛋白被发现参与蛋白分泌功能以及细胞的贴壁生长。拟南芥基因组中有12 个EMP 编码基因,关于它们所编码蛋白质的定位以及功能甚少有研究报道。在此项研究中,借助于不同的生化以及细胞生物学手段,包括瞬时表达、共聚焦成像、电子显微镜分析、pull down 相互作用蛋白捕获以及质谱分析,我将主要研究拟南芥中EMP12 蛋白的亚细胞定位以及分选信号和蛋白靶定机理。通过研究我发现:1)在拟南芥植...
Other Authors: | Gao, Caiji. |
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Format: | Others |
Language: | English Chinese |
Published: |
2012
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Subjects: | |
Online Access: | http://library.cuhk.edu.hk/record=b5549624 http://repository.lib.cuhk.edu.hk/en/item/cuhk-328141 |
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