The functional study of influenza B nucleoprotein.
Lam, Ka Han. === Thesis (M.Phil.)--Chinese University of Hong Kong, 2011. === Includes bibliographical references (leaves 77-82). === Abstracts in English and Chinese. === Acknowledgement --- p.ii === Abstract --- p.iii === 摘要 --- p.v === Content --- p.vii === List of Abbreviations and Symbols -...
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| Format: | Others |
| Language: | English Chinese |
| Published: |
2011
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| Online Access: | http://library.cuhk.edu.hk/record=b5894753 http://repository.lib.cuhk.edu.hk/en/item/cuhk-327523 |
| Summary: | Lam, Ka Han. === Thesis (M.Phil.)--Chinese University of Hong Kong, 2011. === Includes bibliographical references (leaves 77-82). === Abstracts in English and Chinese. === Acknowledgement --- p.ii === Abstract --- p.iii === 摘要 --- p.v === Content --- p.vii === List of Abbreviations and Symbols --- p.xi === Chapter Chapter 1 --- Introduction === Chapter 1.1 --- Severity of influenza --- p.1 === Chapter 1.2 --- Introduction of influenza viruses --- p.3 === Chapter 1.2.1 --- Virion and genome structure --- p.4 === Chapter 1.2.2 --- The replication cycle of influenza viruses --- p.5 === Chapter 1.3 --- Influenza virus NP --- p.8 === Chapter 1.3.1 --- The importance of NP in RNP structure maintenance --- p.9 === Chapter 1.3.2 --- NP self oligomerization --- p.10 === Chapter 1.3.3 --- NP-RNA interaction --- p.12 === Chapter 1.3.4 --- NP and other interacting partners --- p.13 === Chapter 1.4 --- Aim of the project --- p.16 === Chapter Chapter 2 --- Materials and Methods === Chapter 2.1 --- Biological materials --- p.18 === Chapter 2.2 --- Construction of NP mutants --- p.19 === Chapter 2.3 --- Luciferase assay --- p.22 === Chapter 2.4 --- Western blot --- p.23 === Chapter 2.5 --- Protein expression and purification --- p.23 === Chapter 2.6 --- Circular dichroism spectroscopy --- p.24 === Chapter 2.7 --- Static Light scattering --- p.24 === Chapter 2.8 --- Surface plasmon resonance --- p.25 === Chapter 2.9 --- Co-immunoprecipitation (co-IP) --- p.26 === Chapter Chapter 3 --- Identification of residues crucial for NPB oligomerization and ribonucleoprotein activity === Chapter 3.1 --- Introduction --- p.27 === Chapter 3.2 --- Result --- p.31 === Chapter 3.2.1 --- NPB mutants showed deficiency in overall transcription and replication activity --- p.31 === Chapter 3.2.2 --- Expression and purification of NP mutants with low RNP activity --- p.37 === Chapter 3.2.2.1 --- Expression of MBP-tagged NP variants --- p.37 === Chapter 3.2.2.2 --- Purification of MBP-tagged NP variants --- p.38 === Chapter 3.2.3 --- Secondary structures of NP variants were comparable t o wild type NP --- p.41 === Chapter 3.2.4 --- NP variants with low RNP activity were abnormal in oligomerization in vitro --- p.42 === Chapter 3.2.5 --- NP variants with low RNP activity were impaired in homo-oligomer formation in vivo --- p.45 === Chapter 3.2.6 --- Discussion --- p.47 === Chapter Chapter 4 --- Identification of residues crucial for NP 一 RNA interaction and ribonucleoprotein activity === Chapter 4.1 --- Introduction --- p.56 === Chapter 4.2 --- Result --- p.58 === Chapter 4.2.1 --- NPB mutants showed deficiency in overall transcription and replication activity --- p.58 === Chapter 4.2.2 --- Expression and purification of NP variants with low RNP activity --- p.62 === Chapter 4.2.3 --- Secondary structures of NP variants were comparable t o wild type NP --- p.63 === Chapter 4.2.4 --- NP variants with low RNP activity were abnormal in RNA binding --- p.64 === Chapter 4.3 --- Discussion --- p.68 === Chapter Chapter 5 --- Conclusion and future prospect --- p.73 === Copyright --- p.76 === References --- p.77 |
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