The role of human papillomavirus DNA methylation in cervical lesion progression.

• Other Authors: Fung, Man See Joyce.
• Format: Others
• Language: English; Chinese
• Published: 2011
• Subjects: Papillomavirus diseases; DNA > Methylation; Cervix uteri > Cancer; Papillomavirus Infections; DNA Methylation; Uterine Cervical Neoplasms
• Online Access: http://library.cuhk.edu.hk/record=b5894720; http://repository.lib.cuhk.edu.hk/en/item/cuhk-327462

Fung, Man See Joyce. === Thesis (M.Phil.)--Chinese University of Hong Kong, 2011. === Includes bibliographical references (leaves 111-120). === Abstracts in English and Chinese. === Table of Contents === Acknowledgements --- p.I === Abstract --- p.II === 論文摘要 --- p.VII === Table of Contents --- p.X === List of Figures --- p.XIV === List of Tables --- p.XVI === Abbreviations --- p.XVII === Chapter Chapter 1 - --- Introduction --- p.l === Chapter 1.1 --- Biology of HPV --- p.2 === Chapter 1.1.1 --- History --- p.2 === Chapter 1.1.2 --- Classification --- p.2 === Chapter 1.1.3 --- Genome structure --- p.3 === Chapter 1.2 --- HPV and cervical cancer --- p.8 === Chapter 1.2.1 --- Classification of cervical lesions --- p.8 === Chapter 1.2.2 --- Natural history of development of cervical cancer --- p.9 === Chapter 1.2.3 --- Risk factors --- p.11 === Chapter 1.3 --- Prevention of cervical cancer --- p.12 === Chapter 1.3.1 --- Vaccination --- p.12 === Chapter 1.3.2 --- Screening --- p.12 === Chapter 1.3.2.1 --- Pap test --- p.12 === Chapter 1.3.2.2 --- HPV DNA test --- p.13 === Chapter 1.3.2.3 --- Methylation pattern as a novel marker --- p.13 === Chapter 1.4 --- Biology of Methylation --- p.14 === Chapter 1.4.1 --- Definition --- p.14 === Chapter 1.4.2 --- Silencing effect --- p.18 === Chapter 1.4.3 --- Roles in normal development --- p.20 === Chapter 1.5 --- Methylation and human diseases --- p.20 === Chapter 1.5.1 --- Genetic diseases --- p.20 === Chapter 1.5.2 --- Cancers --- p.21 === Chapter 1.5.3 --- Methylation and oncogenic viruses --- p.23 === Chapter 1.5.4 --- Potential of methylation pattern as a novel biomarker of cancer --- p.24 === Chapter 1.5.5 --- Epigenetic therapy --- p.25 === Chapter 1.6 --- Methylation and HPV --- p.25 === Chapter 1.6.1 --- History --- p.25 === Chapter 1.6.2 --- Potential roles in transcription regulation of HPV --- p.26 === Chapter 1.6.3 --- Viral gene methylation --- p.27 === Chapter Chapter 2 - --- "Hypotheses, Objectives and Study Design" --- p.28 === Chapter 2.1 --- Hypotheses --- p.29 === Chapter 2.2 --- Objectives --- p.30 === Chapter 2.3 --- Study Design --- p.30 === Chapter Chapter 3 - --- Materials and Methods --- p.34 === Chapter 3.1 --- Work flow --- p.35 === Chapter 3.2 --- Study subjects --- p.37 === Chapter 3.2.1 --- Invasive cervical cancer group --- p.37 === Chapter 3.2.2 --- Low-grade group --- p.37 === Chapter 3.2.3 --- Cell lines --- p.38 === Chapter 3.3 --- DNA extraction --- p.38 === Chapter 3.4 --- HPV genotyping --- p.39 === Chapter 3.5 --- PCR of HPV16 LCR --- p.39 === Chapter 3.6 --- Sequencing of HPV 16 LCR --- p.42 === Chapter 3.6.1 --- Purification of PCR products --- p.42 === Chapter 3.6.2 --- Cycle sequencing reaction --- p.42 === Chapter 3.6.3 --- Purification of cycle sequencing products --- p.43 === Chapter 3.6.4 --- Sequencer and data analysis --- p.43 === Chapter 3.7 --- Bisulfite modification --- p.43 === Chapter 3.8 --- PCR of bisulfite modified LCR --- p.45 === Chapter 3.9 --- Cloning --- p.48 === Chapter 3.9.1 --- Ligation --- p.48 === Chapter 3.9.2 --- Transformation --- p.48 === Chapter 3.9.3 --- Colony PCR --- p.49 === Chapter 3.10 --- Sequencing of clones --- p.51 === Chapter 3.10.1 --- Purification of PCR products --- p.51 === Chapter 3.10.2 --- Cycle sequencing reaction --- p.51 === Chapter 3.10.3 --- Purification of cycle sequencing products --- p.52 === Chapter 3.10.4 --- Sequencer and data analysis --- p.52 === Chapter 3.11 --- Statistical methods --- p.52 === Chapter Chapter 4 - --- Results --- p.54 === Chapter 4.1 --- Sample selection --- p.55 === Chapter 4.2 --- HPV16 LCR PCR and sequencing --- p.57 === Chapter 4.3 --- Methylation patterns --- p.61 === Chapter 4.3.1 --- Cell lines --- p.61 === Chapter 4.3.2 --- Cancer group --- p.63 === Chapter 4.3.2.1 --- Overview --- p.63 === Chapter 4.3.2.2 --- Methylation pattern of the cancer samples --- p.66 === Chapter 4.3.2.3 --- Methylation pattern of the promoter region --- p.74 === Chapter 4.3.3 --- Low-grade group --- p.76 === Chapter 4.3.3.1 --- Overview --- p.76 === Chapter 4.3.3.2 --- Methylation pattern of the low-grade samples --- p.79 === Chapter 4.3.4 --- Comparison of the methylation patterns of the cancer samples and the low-grade samples --- p.84 === Chapter Chapter 5 - --- Discussion --- p.95 === Chapter 5.1 --- Sequence variations of HPV 16 LCR --- p.96 === Chapter 5.2 --- Methylation patterns of CaSki and SiHa cell lines --- p.98 === Chapter 5.3 --- Methylation pattern of the cancer samples --- p.99 === Chapter 5.4 --- Methylation pattern of the low-grade samples --- p.100 === Chapter 5.5 --- Comparison of methylation patterns of the cancer samples and the low-grade samples --- p.101 === Chapter 5.5.1 --- Promoter region in 3' LCR --- p.102 === Chapter 5.5.1.1 --- SP1 binding site --- p.102 === Chapter 5.5.1.2 --- E2BS3 and E2BS4 --- p.103 === Chapter 5.5.2 --- Silencer region --- p.104 === Chapter 5.5.3 --- Enhancer region in central LCR --- p.105 === Chapter 5.5.4 --- CpG sites within 5' LCR --- p.106 === Chapter 5.6 --- Role of methylation in HPV 16 --- p.107 === Chapter 5.7 --- Potential as novel biomarker --- p.108 === Chapter 5.8 --- Conclusions --- p.109 === References --- p.111 === Appendix A