Activation of human eosinophils by novel t-helper type 2 cytokine IL-33: implications for the immunopathology of allergic inflammation.

• Other Authors: Chow, Yin Sau Joyce.
• Format: Others
• Language: English; Chinese
• Published: 2009
• Subjects: Inflammation; Allergy; Eosinophils; Cytokines; Inflammation > pathology; Inflammation > immunology; Hypersensitivity > pathology; Hypersensitivity > immunology; Eosinophils > pathology
• Online Access: http://library.cuhk.edu.hk/record=b5893986; http://repository.lib.cuhk.edu.hk/en/item/cuhk-326936

Chow, Yin Sau Joyce. === Thesis (M.Phil.)--Chinese University of Hong Kong, 2009. === Includes bibliographical references (leaves 127-140). === Abstract also in Chinese. === Abstract --- p.I === 摘要 --- p.V === Acknowledgements --- p.VIII === Publications --- p.X === Table of contents --- p.XII === Abbreviations --- p.XV === Chapter Chapter 1: --- General Introduction --- p.1 === Chapter 1.1 --- Allergic diseases and allergic inflammation --- p.1 === Chapter 1.1.1 --- Allergic diseases and their prevalence --- p.1 === Chapter 1.1.2 --- Immunopathology of allergic inflammation --- p.2 === Chapter 1.2 --- Biology of human eosinophils --- p.4 === Chapter 1.2.1 --- Morphology --- p.4 === Chapter 1.2.2 --- Cell surface receptors and mediators --- p.4 === Chapter 1.2.3 --- Origin and development of eosinophils --- p.7 === Chapter 1.3 --- Eosinophils and allergic inflammation --- p.7 === Chapter 1.3.1 --- Adhesion molecules on eosinophils for emigration --- p.8 === Chapter 1.3.2 --- Eosinophil activation and inflammatory mediators --- p.13 === Chapter 1.3.3 --- Survival of eosinophils in allergic inflammation --- p.18 === Chapter 1.3.4 --- Immunopathological roles of eosinophils in allergic inflammation --- p.18 === Chapter 1.4 --- Intracellular signaling mechanisms --- p.20 === Chapter 1.4.1 --- Signal transduction pathways of eosinophils --- p.20 === Chapter 1.4.2 --- Inhibitors of signaling molecules --- p.26 === Chapter 1.5 --- Aim of study --- p.29 === Chapter Chapter 2: --- Materials and Methods --- p.31 === Chapter 2.1 --- Materials --- p.31 === Chapter 2.1.1 --- Human eosinophils --- p.31 === Chapter 2.1.2 --- Cell culture --- p.33 === Chapter 2.1.3 --- Cell surface and intracellular immunofluorescent staining --- p.36 === Chapter 2.1.4 --- Detection of cytokine and chemokine release --- p.39 === Chapter 2.1.5 --- Detection of cell viability and apoptosis --- p.40 === Chapter 2.1.6 --- Protein extraction --- p.40 === Chapter 2.1.7 --- Western blot analysis --- p.40 === Chapter 2.1.8 --- Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) --- p.42 === Chapter 2.2 --- Methods --- p.45 === Chapter 2.2.1 --- Purification of human eosinophils --- p.45 === Chapter 2.2.2 --- Cell culture --- p.46 === Chapter 2.2.3 --- Cell surface and intracellular immunofluorescent staining --- p.47 === Chapter 2.2.4 --- Detection of cytokine and chemokine release --- p.48 === Chapter 2.2.5 --- Detection of cell viability and apoptoas --- p.49 === Chapter 2.2.6 --- Protein extraction --- p.49 === Chapter 2.2.7 --- Western blot analysis --- p.50 === Chapter 2.2.8 --- SDS-PAGE --- p.50 === Chapter 2.2.9 --- Statistical analysis --- p.51 === Chapter Chapter 3: --- Role of Novel IL-1 Family Cytokine in Allergic Inflammation: IL-33-mediated Eosinophil Activation --- p.52 === Chapter 3.1 --- Introduction --- p.52 === Chapter 3.2 --- Results --- p.54 === Chapter 3.2.1 --- "Total protein expression of IL-33 receptor, ST2, of human eosinophils" --- p.54 === Chapter 3.2.2 --- "Intracellular protein expression of IL-33 receptor, ST2，in human eosinophils" --- p.55 === Chapter 3.2.3 --- "Extracellular protein expression of IL-33 receptor, ST2, on human eosinophils" --- p.56 === Chapter 3.2.4 --- "Effects of IL-1β IL-18, and IL-33 on survival of human eosinophils" --- p.57 === Chapter 3.2.5 --- "Effects of IL-1β, IL-18, and DL-33 on surface adhesion molecule expression on human eosinophils" --- p.60 === Chapter 3.2.6 --- "Effects of IL-1β, IL-18, and IL-33 on chemokine and cytokine release from human eosinophils" --- p.64 === Chapter 3.2.7 --- "Synergistic effects of IL-1β, IH8, and IL-33 on IL-6 release from human eosinophils" --- p.69 === Chapter 3.2.8 --- "Effects of transcription and translation inhibitors on IL- 1β, IL-18, and IL-33-induced chemokine and cytokine release from human eosinophils" --- p.71 === Chapter 3.2.9 --- "Effects of different inhibitors on lL-1β, IL-18, and IL-33-induced survival enhancement of human eosinophils" --- p.75 === Chapter 3.2.10 --- Effects of different inhibitors on IL-1β and IL-33-mediated surface expression of adhesion molecules on human eosinophils --- p.77 === Chapter 3.2.11 --- "Effects of different inhibitors on IL-33, IL-1β，and IL-18-induced release of CCL2，CXCL8，and IL-6 from human eosinophils" --- p.79 === Chapter 3.2.12 --- "Effects of IL-33, IL-1β and IL-18 on activation of ERK, p38 MAPK, and NF-kB pathways in human eosinophils" --- p.83 === Chapter 3.3 --- Discussion --- p.85 === Chapter Chapter 4: --- Co-culture of Eosinophils & Epidermal Keratinocytes Upon IL-33 Stimulation: Implications for Immunopathology of Atopic Dermatitis --- p.95 === Chapter 4.1 --- Introduction --- p.95 === Chapter 4.2 --- Results --- p.98 === Chapter 4.2.1 --- Effect of IL-33 on surface expression of CD18 and ICAM-1 upon the interaction of human eosinophils and epidermal keratinocytes --- p.98 === Chapter 4.2.2 --- Effect of IL-33 on CCL2 release upon the interaction of human eosinophils and epidermal keratinocytes --- p.98 === Chapter 4.2.3 --- Effect of IL-33 on CXCL8 release upon the interaction of human eosinophils and epidermal keratinocytes --- p.101 === Chapter 4.2.4 --- Effect of IL-33 on IL-6 release upon the interaction of human eosinophils and epidermal keratinocytes --- p.101 === Chapter 4.2.5 --- Source(s) of CCL2 in co-culture of human eosinophils and epidermal keratinocytes upon IL-33 stimulation --- p.104 === Chapter 4.2.6 --- Source(s) of CXCL8 in co-culture of human eosinophils and epidermal keratinocytes upon IL-33 stimulation --- p.105 === Chapter 4.2.7 --- Source(s) of IL-6 in co-culture of human eosinophils and epidermal keratinocytes upon BL-33 stimulation --- p.108 === Chapter 4.2.8 --- "Effect of transwell insert on the induction of CCL2，CXCL8, and IL-6 release in co-culture of human eosinophils and epidermal keratinocytes upon IL-33 stimulation" --- p.110 === Chapter 4.3 --- Discussion --- p.113 === Chapter Chapter 5: --- Concluding Remarks and Future Perspectives --- p.120 === Chapter 5.1 --- Concluding Remarks --- p.120 === Chapter 5.2 --- Future Perspectives --- p.123 === Appendix --- p.126 === References --- p.127