Analysis of anti-proliferation activities of drought tolerant soybean lines.

• Other Authors: Yuen, Ka Leung.
• Format: Others
• Language: English; Chinese
• Published: 2009
• Subjects: Cancer > Treatment; Isoflavones > Health aspects; Isoflavones > Synthesis; Soybean; Neoplasms > drug therapy; Isoflavones > therapeutic use; Isoflavones > biosynthesis; Soybeans
• Online Access: http://library.cuhk.edu.hk/record=b5894079; http://repository.lib.cuhk.edu.hk/en/item/cuhk-326768

Yuen, Ka Leung. === Thesis (M.Phil.)--Chinese University of Hong Kong, 2009. === Includes bibliographical references (leaves 95-104). === Abstracts in English and Chinese. === Chapter 1 --- Introduction === Chapter 1.1 --- CANCER === Chapter 1.1.1 --- OVERVIEW OF CANCER --- p.1 === Chapter 1.1.2 --- DEVELOPMENT OF CANCER --- p.1 === Chapter 1.1.3 --- CHARACTERISTICS OF CANCER CELLS --- p.3 === Chapter 1.1.4 --- CATEGORIZATION OF CANCER --- p.6 === Chapter 1.1.5 --- RISK FACTORS IN CANCER DEVELOPMENT --- p.7 === Chapter 1.1.6 --- EPIDEMIOLOGY OF CANCER --- p.11 === Chapter 1.1.7 --- CANCER THERAPIES --- p.13 === Chapter 1.2 --- SOYBEANS AND ISOFLAVONES === Chapter 1.2.1 --- GENERAL INTRODUCTION OF ISOFLAVONES --- p.18 === Chapter 1.2.2 --- NATURAL FUNCTIONS OF ISOFLAVONES --- p.19 === Chapter 1.2.3 --- STRUCTURES OF ISOFLAVONES --- p.19 === Chapter 1.2.4 --- BIOACTIVITIES OF SOY ISOFLAVONES --- p.20 === Chapter 1.2.5 --- PRODUCTION OF SOY ISOFLAVONES CAN BE AFFECTED BY MANY FACTORS --- p.21 === Chapter 1.3 --- THE AIM AND OBJECTIVES OF THE PROJECT === Chapter 1.3.1 --- AIM OF THE PROJECT --- p.22 === Chapter 1.3.2 --- OBJECTIVES OF THE PROJECT --- p.23 === Chapter 2 --- Materials === Chapter 2.1 --- 19 DROUGHT TOLERANT SOYBEAN LINES --- p.24 === Chapter 2.2 --- 5 HUMAN CANCER CELL LINES --- p.25 === Chapter 2.3 --- CHEMICALS --- p.25 === Chapter 2.4 --- REAGENTS --- p.26 === Chapter 2.5 --- SOLUTIONS --- p.26 === Chapter 2.6 --- MAJOR EQUIPMENTS AND MATERIALS --- p.28 === Chapter 3 --- Methodology === Chapter 3.1 --- PREPARATION OF SOYBEAN EXTRACTS --- p.29 === Chapter 3.2 --- HIGH PERFORMANCE LIQUID CHROMATOGRAPHY(HPLC) ANALYSIS OF SOYBEAN EXTRACTS === Chapter 3.2.1 --- PREPARATION OF SOYBEAN EXTRACTS FOR HPLC ANALYS --- p.30 === Chapter 3.2.2 --- HPLC ANALYSIS --- p.30 === Chapter 3.3 --- PREPARATION OF 5 HUMAN CANCER CELL LINES FOR ANTI-PROLIFERATION ASSAY === Chapter 3.3.1 --- THAWING OF THE C Y R O P R E S E R V E D CELL LINES --- p.31 === Chapter 3.3.2 --- MAINTAINING OF CELL LINES --- p.32 === Chapter 3.3.3 --- ANTI-PROLIFERATION TEST WITH MTT ASSAY --- p.33 === Chapter 3.4 --- STATISTICS --- p.35 === Chapter 4 --- Results === Chapter 4.1 --- PREPARARTION OF SOYBEAN EXTRACTS --- p.36 === Chapter 4.2 --- HPLC ANALYSIS OF 5 SELECTED ISOFLAVONES IN 19 SOYBEAN SAMPLES --- p.36 === Chapter 4.3 --- COMPARISON OF SUM OF 5 SELECTED ISOFLAVONES FROM THE PARENT SOYBEAN AND VARIETIES HARVESTED FROM IRRIGATED LAND --- p.43 === Chapter 4.4 --- COMPARISON OF SUM OF 5 SELECTED ISOFLAVONES FROM THE SOYBEAN VARIETIES HARVESTED FROM DROUGHT LAND --- p.45 === Chapter 4.5 --- COMPARISON OF SELECTED ISOFLAVONES FROM THE PARENT SOYBEAN AND VARIETIES HARVESTED FROM IRRIGATED LAND --- p.47 === Chapter 4.6 --- COMPARISON OF SELECTED ISOFLAVONES FROM THE PARENT SOYBEAN AND VARIETIES HARVESTED FROM DAROUGHT LAND --- p.54 === Chapter 4.7 --- COMPARISON OF SUM OF SELECTED ISOFLAVONES AMONG THE SOYBEANS HARVESTED FROM IRRIGATED LAND AND DROUGHT LAND --- p.59 === Chapter 4.8 --- DETERMINATION OF ANTI-PROLIFERATION ABILITIES OF SOYBEAN SAMPLES --- p.19 === Chapter 4.8.1 --- ANTI-PROLIFERATION TEST OF ETHANOL AND 2-PHEN YLCHROMONE --- p.61 === Chapter 4.8.2 --- ANTI-PROLIFERATION ACTIVITIES OF 19 SOYBEAN SAMPLES ON 5 HUMAN CANCER CELL LINES --- p.61 === Chapter 4.9 --- COMPARISON OF ANTI-PROLIFERATION POTENCIES OF19 SOYBEAN SAMPLES WITH SUM OF SELECTED ISOFLAVONES --- p.70 === Chapter 4.10 --- COMPARISON OF ANTI-PROLIFERATION POTENCIES OF19 SOYBEAN SAMPLES --- p.72 === Chapter 4.11 --- ANTI-PROLIFERATION EFFECT OF INDIVIDUAL ISOFLAVONES ON FIVE CANCER CELL LINES --- p.74 === Chapter 5 --- Discussion === Chapter 5.1 --- EXTRACTION OF 19 SOYBEAN LINES --- p.77 === Chapter 5.2 --- DETERMINATION OF QUANTITIES OF SELECTED ISOFLAVONES IN 19 SOYBEAN SAMPLES BY HPLC ANALYSIS --- p.77 === Chapter 5.3 --- COMPARISON OF SELECTED ISOFLAVONES AMONG 19 SOYBEAN SAMPLES === Chapter 5.3.1 --- COMPARISON OF SUM OF SELECTED ISOFLAVONES BETWEEN PARENT AND SOYBEANS HARVESTED FROM IRRIGATED LAND --- p.80 === Chapter 5.3.2 --- COMPARISON OF SUM OF SELECTED ISOFLAVONES BETWEEN SOYBEANS HARVESTED FROM DROUGHT LAND --- p.81 === Chapter 5.3.3 --- COMPARISON OF SELECTED ISOFLAVONES BETWEEN SOYBEANS HARVESTED FROM IRRIGATED LAND --- p.81 === Chapter 5.3.4 --- COMPARISON OF SELECTED ISOFLAVONES BETWEEN SOYBEANS HARVESTED FROM DROUGHT LAND --- p.82 === Chapter 5.3.5 --- COMPARISON OF SUM OF SELECTED ISOFLAVONES BETWEEN SOYBEANS HARVESTED FROM IRRIGATED LAND AND DROUGHT LAND --- p.83 === Chapter 5.4 --- COMPARISON OF ANTI-PROLIFERATION ACTIVITIES OF 19 SOYBEAN SAMPLES === Chapter 5.4.1 --- COMPARISON OF ANTI-PROLIFERATION ACTIVITIES OF19 SOYBEAN SAMPLES AMONG 5 CANCER CELL LINES --- p.84 === Chapter 5.4.2 --- COMPARISON OF ANTI-PROLIFERATION POTENCIES OF19 SOYBEAN SAMPLES --- p.85 === Chapter 5.4.3 --- COMPARISON OF ANTI-PROLIFERATION ACTIVITIES OF19 SOYBEAN SAMPLES AND CORRESPONDING SUM OF SELECTED ISOFLAVONES --- p.86 === Chapter 5.4.4 --- COMPARISON OF IC50S FROM SOYBEANS HARVESTED FROM IRRIGATED LAND AND DROUGHT LAND --- p.87 === Chapter 5.4.5 --- CORRELATION OF ISOFLAVONES AND ANTI-PROLIFERATION POTENCIES --- p.88 === Chapter 6 --- Conclusion --- p.90 === Chapter 7 --- References --- p.91 === Chapter 8 --- Appendix --- p.S1