Transient cell cycle arrest and autophagy induction in colorectal cancer HT29 cell line by sodium 5,6-benzylidene-L-ascorbate.

• Other Authors: Cheung, Wing Ki.
• Format: Others
• Language: English; Chinese
• Published: 2008
• Subjects: Colon (Anatomy) > Cancer > Treatment; Rectum > Cancer > Treatment; Colorectal Neoplasms > therapy; HT29 Cells > cytology; Ascorbic Acid; Benzylidene Compounds
• Online Access: http://library.cuhk.edu.hk/record=b5893623; http://repository.lib.cuhk.edu.hk/en/item/cuhk-326564

Cheung, Wing Ki. === Thesis (M.Phil.)--Chinese University of Hong Kong, 2008. === Includes bibliographical references (leaves 100-112). === Abstracts in English and Chinese. === Acknowledgments === Abbreviations === Abstract 一 English --- p.i === - Chinese --- p.iii === Chapter Chapter 1 --- General Introduction === Chapter 1.1. --- Colon Cancer === Chapter 1.1.1. --- Colon cancer statistic in Hong Kong --- p.1 === Chapter 1.1.2. --- Development of Colon cancer --- p.1 === Chapter 1.1.3. --- Treatment --- p.2 === Chapter 1.2. --- Chemistry of ascorbates === Chapter 1.2.1. --- Sodium-L-ascorbate --- p.3 === Chapter 1.2.2. --- "Sodium 5,6-benazylidene-L-ascorbate" --- p.4 === Chapter 1.3. --- "Reactive oxygen species and reactive nitrogen species, and their biological consequences" --- p.5 === Chapter 1.4. --- Cell cycle --- p.7 === Chapter 1.5. --- Autophagy --- p.8 === Chapter 1.6. --- Human colon cancer HT29 cells for anti-tumor study --- p.9 === Chapter 1.7 --- Aim of study --- p.10 === Chapter Chapter 2 --- Comparative studies of cytotoxicity of SAA and SBA in short term treatment === Chapter 2.1. --- Introduction --- p.11 === Chapter 2.2. --- Materials and Methods --- p.14 === Chapter 2.3. --- Results --- p.17 === Chapter 2.4. --- Discussion --- p.26 === Chapter Chapter 3 --- Comparative studies of SAA and SBA in oxidative stress induction and their corresponding ROS inhibitors === Chapter 3.1. --- Introduction --- p.28 === Chapter 3.2. --- Materials and Methods --- p.31 === Chapter 3.3. --- Results --- p.35 === Chapter 3.4. --- Discussion --- p.42 === Chapter Chapter 4 --- "Effects of SAA and SBA treatments on cell cycle regulatory proteins and the induction of transient cell cycle arrests in Gl, S and G2 phases Cell Cycle" === Chapter 4.1. --- Introduction --- p.45 === Chapter 4.2. --- Materials and Methods --- p.49 === Chapter 4.3. --- Results --- p.53 === Chapter 4.4. --- Discussion --- p.69 === Chapter Chapter 5 --- Autophagy induction during SBA treatment and autophagy inhibition during SAA treatment === Chapter 5.1. --- Introduction --- p.72 === Chapter 5.2. --- Materials and Methods --- p.74 === Chapter 5.3. --- Results --- p.77 === Chapter 5.4. --- Discussion --- p.91 === Chapter Chapter 6 --- General Discussion --- p.93 === References --- p.100