DNA-quantum dot molecular opto-electronic switch with combined Förster resonance energy transfer and photovoltaic effect for accurate DNA recognition.

• Other Authors: Qi, Huijie.
• Format: Others
• Language: English; Chinese
• Published: 2008
• Subjects: Quantum dots; DNA fingerprinting; Energy transfer; Molecular dynamics; Optoelectronics
• Online Access: http://library.cuhk.edu.hk/record=b5896846; http://repository.lib.cuhk.edu.hk/en/item/cuhk-326508

Qi, Huijie = 用于脫氧核糖核酸分子精确识别的Förster共振能量转移与光电压效应相结合的DNA-量子点分子光电开关 / 齐慧杰. === Thesis (M.Phil.)--Chinese University of Hong Kong, 2008. === Includes bibliographical references. === Abstracts in English and Chinese. === Qi, Huijie = Yong yu tuo yang he tang he suan fen zi jing que shi bie de Förster gong zhen neng liang zhuan yi yu guang dian ya xiao ying xiang jie he de DNA-liang zi dian fen zi guang dian kai guan / Qi Huijie. === Abstract --- p.i === Acknowledgements --- p.iv === Table of contents --- p.v === List of Figures --- p.ix === List of Tables --- p.xiii === Chapter Chapter 1 --- Introduction --- p.1 === Chapter 1.1 --- Background --- p.1 === Chapter 1.1.1 --- Nanotechnology and nanomaterials --- p.1 === Chapter 1.1.2 --- "Semiconductor quantum dots: optical properties, preparation and applications" --- p.3 === Chapter 1.1.2.1 --- Preparation --- p.3 === Chapter 1.1.2.2 --- Applications --- p.5 === Chapter 1.1.3 --- Quantum dot-based DNA recognition --- p.10 === Chapter 1.1.3.1 --- Forster resonance energy transfer --- p.11 === Chapter 1.1.3.2 --- Bioimmobilization technique --- p.13 === Chapter 1.1.3.3 --- Highlights on Quantum dot-based DNA recognition --- p.14 === Chapter 1.2 --- Objective and methodology --- p.16 === Chapter 1.2.1 --- Objective --- p.16 === Chapter 1.2.2 --- General methodology --- p.17 === References --- p.19 === Chapter Chapter 2 --- Instrumentation --- p.23 === Chapter 2.1 --- Introduction --- p.23 === Chapter 2.2 --- Atomic Force Microscopy --- p.23 === Chapter 2.2.1 --- Principle of Atomic Force Microscopy --- p.24 === Chapter 2.2.2 --- Instrumentation --- p.29 === Chapter 2.3 --- Absorption and Fluorescence spectroscopy --- p.30 === Chapter 2.3.1 --- Basic Principle --- p.30 === Chapter 2.3.2 --- Instrumentation --- p.33 === Chapter 2.4 --- I-V characteristic --- p.36 === Chapter 2.4.1 --- Basic principle --- p.36 === Chapter 2.4.2 --- Instrumentation --- p.36 === Chapter 2.5 --- Other instrumentations --- p.37 === References --- p.38 === Chapter Chapter 3 --- Preparation of DNA/QD network systems --- p.39 === Chapter 3.1 --- Introduction --- p.39 === Chapter 3.2 --- Preparation of DNA/QD network conjugates in solution --- p.39 === Chapter 3.2.1 --- Experimental --- p.39 === Chapter 3.2.2 --- Characterization --- p.41 === Chapter 3.3 --- Preparation of DNA/QD network on substrates --- p.44 === Chapter 3.3.1 --- Experimental --- p.44 === Chapter 3.3.2 --- Characterization --- p.46 === Chapter 3.4 --- Summary --- p.49 === References --- p.50 === Chapter Chapter 4 --- Fluorescence and I-V characteristics for DNA/QD systems --- p.51 === Chapter 4.1 --- Introduction --- p.51 === Chapter 4.2 --- Experimental --- p.52 === Chapter 4.2.1 --- Patterned Au electrodes --- p.52 === Chapter 4.2.2 --- Electric field induced assembly --- p.52 === Chapter 4.3 --- Results and Discussion --- p.55 === Chapter 4.3.1 --- Optical studies of DNA/QD systems --- p.55 === Chapter 4.3.1.1 --- Optical characteristics of QDs used --- p.55 === Chapter 4.3.1.2 --- Optical studies of DNA/QD network systems in solution --- p.56 === Chapter 4.3.1.3 --- Optical studies of DNA cross-linked QD monolayer on substrates --- p.58 === Chapter 4.3.2 --- PV characteristics --- p.62 === Chapter 4.4 --- Summary --- p.64 === References --- p.66 === Chapter Chapter 5 --- DNA-quantum dot molecular opto-electronic switch with combined Forster resonance energy transfer and photovoltaic effect --- p.68 === Chapter 5.1 --- Introduction --- p.68 === Chapter 5.2 --- Experimental --- p.69 === Chapter 5.2.1 --- Preparation of DNA/QD molecular switch in solution --- p.69 === Chapter 5.2.2 --- Preparation of platform of molecular optoelectronic switch --- p.72 === Chapter 5.2.3 --- EFIA --- p.73 === Chapter 5.3 --- Results and Discussion --- p.73 === Chapter 5.3.1 --- Optical studies of molecular switch constructed of DNA/QD/Cy5 system --- p.73 === Chapter 5.3.1.1 --- Studies on optical properties of molecular switch as well as sole FRET effect between QD and Cy5in solution --- p.73 === Chapter 5.3.1.2 --- Optical studies of dsDNAs cross-linked QD monolayer on substrates with presence of Cy5 --- p.79 === Chapter 5.3.2 --- Studies on FRET mediated PV effect of DNA/QD/Cy5 molecular optoelectronic switch --- p.80 === Chapter 5.4 --- Summary --- p.82 === References --- p.83 === Chapter Chapter 6 --- Conclusions and Future Work --- p.84 === Chapter 6.1 --- Conclusions --- p.84 === Chapter 6.2 --- Future work --- p.85