Developmental role of the S100A1 protein.

• Other Authors: Cheung, Siu Yuen.
• Format: Others
• Language: English; Chinese
• Published: 2008
• Subjects: Embryology; Calcium-binding proteins; Calcium-Binding Proteins; S100 Proteins
• Online Access: http://library.cuhk.edu.hk/record=b5893553; http://repository.lib.cuhk.edu.hk/en/item/cuhk-326506

Cheung, Siu Yuen. === Thesis (M.Phil.)--Chinese University of Hong Kong, 2008. === Includes bibliographical references (leaves 178-200). === Abstracts in English and Chinese. === Abstract --- p.i === Chinese abstract --- p.iii === Acknowledgements --- p.v === Table of contents --- p.vii === Chapter Chapter One --- General Introduction --- p.1 === Chapter 1.1 --- S100 Proteins --- p.1 === Chapter 1.1.1 --- Structure of S100 proteins --- p.2 === Chapter 1.1.2 --- Possible functions of S100 proteins --- p.4 === Chapter 1.1.3 --- Genomic organization of S100 genes --- p.6 === Chapter 1.1.4 --- Clinical importance of S100 proteins --- p.7 === Chapter 1.2 --- S100A1 Protein --- p.8 === Chapter 1.2.1 --- Possible functions of the S100A1 protein --- p.10 === Chapter 1.2.1.1 --- Regulation of cardiac and skeletal muscle contractility --- p.10 === Chapter 1.2.1.2 --- Functional roles in the central nervous system (CNS) --- p.12 === Chapter 1.2.1.3 --- Other possible functions of the S100A1 protein --- p.13 === Chapter 1.2.2 --- S100A1 knockout mice --- p.14 === Chapter 1.2.3 --- Relationships between S100A1 and S100B proteins --- p.16 === Chapter 1.3 --- S100B Protein --- p.18 === Chapter 1.3.1 --- Possible functions of S100B protein --- p.19 === Chapter 1.3.2 --- S100B knockout mice --- p.20 === Chapter 1.4 --- RNA interference --- p.22 === Chapter 1.4.1 --- Mechanisms of RNA interference --- p.24 === Chapter 1.4.2 --- Efficacy and selectivity of siRNA --- p.25 === Chapter 1.4.3 --- siRNA delivery --- p.27 === Chapter 1.5 --- Objective --- p.31 === Figures and legends --- p.34 === Chapter Chapter Two --- S100A1 expression in normal mouse embryos and characterization of S100A1 knockout mouse embryos --- p.40 === Chapter 2.1 --- Introduction --- p.40 === Chapter 2.2 --- Materials and Methods --- p.44 === Chapter 2.2.1 --- Mouse strains --- p.44 === Chapter 2.2.2 --- RNA extraction --- p.46 === Chapter 2.2.3 --- Reverse Transcription-Polymerase Chain Reaction (RT-PCR) --- p.46 === Chapter 2.2.4 --- Protein extraction --- p.48 === Chapter 2.2.5 --- Western blotting --- p.49 === Chapter 2.2.6 --- Immunohistochemical staining --- p.50 === Chapter 2.3 --- Results --- p.53 === Chapter 2.3.1 --- S100A1 mRNA expression in normal mouse embryo --- p.53 === Chapter 2.3.2 --- S100A1 protein expression in normal mouse embryos --- p.55 === Chapter 2.3.2.1 --- Temporal expression of the S100A1 protein --- p.55 === Chapter 2.3.2.2 --- Spatial expression of the S100A1 protein --- p.57 === Chapter 2.3.3 --- Morphological and histological characterization of SI00A1 knockout mouse embryos --- p.60 === Chapter 2.3.4 --- S100B protein expression pattern in Wt and S100A1 KO mouse embryos --- p.62 === Chapter 2.4 --- Discussion --- p.64 === Tables --- p.73 === Figures and legends --- p.76 === Chapter Chapter Three --- Knockdown of S100A1 in S100B in knockout mouse embryos --- p.118 === Chapter 3.1 --- Introduction --- p.118 === Chapter 3.2 --- Materials and Methods --- p.128 === Chapter 3.2.1 --- Mouse strains --- p.128 === Chapter 3.2.2 --- Short-interfering RNA (siRNA) --- p.129 === Chapter 3.2.3 --- In-uterus surgery --- p.130 === Chapter 3.2.4 --- RNA extraction and RT-PCR --- p.132 === Chapter 3.2.5 --- Immunohistochemical staining of S100A1 and S100B --- p.132 === Chapter 3.3 --- Results --- p.133 === Chapter 3.3.1 --- Characterization of S100B knockout mouse embryos --- p.133 === Chapter 3.3.2 --- S100A1 knockdown in S100B wild-type (Wt) mouse embryos --- p.133 === Chapter 3.3.3 --- S100A1 knockdown in S100B knockout (KO) mouse embryos --- p.139 === Chapter 3.4 --- Discussion --- p.146 === Tables --- p.153 === Figures and legends --- p.154 === Chapter Chapter Four --- General Discussion and Conclusions --- p.175 === Reference --- p.178