Some studies on the effects of oestrogens on mast cells.

• Other Authors: Law, Ka Yan Jessica.
• Format: Others
• Language: English; Chinese
• Published: 2007
• Subjects: Estrogen; Mast cells; Estrogens; Mast Cells
• Online Access: http://library.cuhk.edu.hk/record=b5896773; http://repository.lib.cuhk.edu.hk/en/item/cuhk-326209

Law, Ka Yan Jessica. === Thesis (M.Phil.)--Chinese University of Hong Kong, 2007. === Includes bibliographical references (leaves 194-203). === Abstracts in English and Chinese. === Abstract (English) --- p.i === Abstract (Chinese) --- p.iv === Acknowledgements --- p.vi === Publications --- p.vii === Abbreviations --- p.viii === Chapter 1. --- Introduction --- p.1 === Chapter 1.1 --- Mast Cell --- p.1 === Chapter 1.1.1 --- Origin of Mast Cell --- p.2 === Chapter 1.1.2 --- Heterogeneity of Mast Cell --- p.3 === Chapter 1.1.3 --- Activation of Mast Cells --- p.5 === Chapter 1.1.3.1 --- IgE-dependent Mast cell Activation --- p.5 === Chapter 1.1.3.2 --- IgE-independent Mast Cell Activation --- p.10 === Chapter 1.1.3.3 --- Importance of Calcium --- p.12 === Chapter 1.1.3.4 --- "Role of cyclic adenosine ´3ة,5'-monophosphate (cAMP)" --- p.15 === Chapter 1.1.4 --- Mast Cells Mediators --- p.16 === Chapter 1.1.5 --- Physiological and Pathological Roles of Mast Cells --- p.19 === Chapter 1.2 --- Oestrogens --- p.22 === Chapter 1.2.1 --- Oestrogens and Inflammation --- p.23 === Chapter 1.2.1.1 --- Pro-inflammatory Effects of Oestrogen --- p.23 === Chapter 1.2.1.2 --- Anti-inflammatory Effects of Oestrogen --- p.24 === Chapter 1.2.2 --- Oestrogen Signaling --- p.25 === Chapter 1.2.2.1 --- Classical Genomic Pathway --- p.26 === Chapter 1.2.2.2 --- Non-genomic Pathway --- p.30 === Chapter 1.3 --- Aims of Study --- p.34 === Chapter 2. --- Materials and Methods --- p.36 === Chapter 2.1 --- Materials --- p.36 === Chapter 2.1.1 --- Drugs --- p.36 === Chapter 2.1.1.1 --- Oestrogen Receptor Agonists and Antagonists --- p.36 === Chapter 2.1.1.2 --- DNA Transcription and Translation Blockers --- p.41 === Chapter 2.1.1.3 --- Mast Cell Secretagogues --- p.41 === Chapter 2.1.2 --- Materials for Histamine Assay Experiments --- p.41 === Chapter 2.1.2.1 --- Materials for Rat Sensitization --- p.41 === Chapter 2.1.2.2 --- Materials for Ovariectomy surgery --- p.42 === Chapter 2.1.2.3 --- Materials for Buffers --- p.42 === Chapter 2.1.2.4 --- Materials for Histamine Release Assay --- p.43 === Chapter 2.1.2.5 --- Miscellaneous --- p.43 === Chapter 2.1.3 --- Materials for Oestrogen Receptors Identification --- p.44 === Chapter 2.1.3.1 --- Oestrogen Receptors Expression --- p.44 === Chapter 2.1.3.1.1 --- Materials for RNA Extraction --- p.44 === Chapter 2.1.3.1.2 --- Materials for cDNA Construction by RT- PCR --- p.45 === Chapter 2.1.3.1.3 --- Materials for PCR --- p.45 === Chapter 2.1.3.1.4 --- Materials for Agarose Gel Electrophoresis --- p.46 === Chapter 2.1.3.1.5 --- Miscellaneous --- p.46 === Chapter 2.2 --- Histamine Assay Experiments --- p.47 === Chapter 2.2.1 --- Preparation of Buffers --- p.47 === Chapter 2.2.2 --- Preparation of Stock Solutions --- p.48 === Chapter 2.2.2.1 --- Preparation of Mast Cell Secretagogues Stock Solutions --- p.48 === Chapter 2.2.2.2 --- Preparation of Oestrogens Stock Solutions --- p.48 === Chapter 2.2.3 --- Animals and Rat Peritoneal Mast Cell Isolation --- p.49 === Chapter 2.2.3.1 --- Animals --- p.49 === Chapter 2.2.3.2 --- Rats Sensitization --- p.49 === Chapter 2.2.3.3 --- Ovariectomy Surgery --- p.49 === Chapter 2.2.3.4 --- Isolation of Rat Peritoneal Mast Cells --- p.50 === Chapter 2.2.3.5 --- Purification of Mast Cells --- p.51 === Chapter 2.2.3.6 --- Determination of Mast Cell Number and Viability --- p.51 === Chapter 2.2.4 --- Histamine Release Assay and Histamine Measurement --- p.52 === Chapter 2.2.4.1 --- Histamine Release Assay --- p.52 === Chapter 2.2.4.2 --- Fluorescence Assay of Histamine --- p.53 === Chapter 2.2.5 --- Calculation of Histamine Release Level and Inhibition --- p.54 === Chapter 2.2.6 --- Statistics Analysis --- p.55 === Chapter 2.3 --- Oestrogen Receptors Identification --- p.55 === Chapter 2.3.1 --- Oestrogen Receptors Expression --- p.55 === Chapter 2.3.1.1 --- Preparation of Buffers --- p.55 === Chapter 2.3.1.2 --- Isolation of Mast Cell mRNA --- p.56 === Chapter 2.3.1.3 --- Heparinase Treatment --- p.57 === Chapter 2.3.1.4 --- Construction of Mast Cell cDNA by RT-PCR --- p.57 === Chapter 2.3.1.5 --- Amplification of Oestrogen receptors cDNA by PCR method --- p.58 === Chapter 2.3.1.6 --- Agarose Gel Electrophoresis --- p.58 === Chapter 3. --- Effects of Oestrogens on Immunological Induced Histamine Release from Rat Peritoneal Mast Cells --- p.59 === Chapter 3.1 --- Introduction --- p.59 === Chapter 3.2 --- Materials and Methods --- p.62 === Chapter 3.3 --- Results --- p.63 === Chapter 3.3.1 --- Activation of RPMCs by Immunological Stimuli --- p.63 === Chapter 3.3.1.1 --- Effects of Oestrogen Receptor Agonists on Histamine Release from RPMCs --- p.63 === Chapter 3.3.1.2 --- Effects of Oestrogen on Anti-IgE mediated Histamine Release from RPMCs --- p.64 === Chapter 3.3.1.3 --- Effects of Selective Oestrogen Receptor Modulators on Anti-IgE Mediated Histamine Release from RPMCs --- p.65 === Chapter 3.3.1.4 --- Effects of Selective Oestrogen Agonists on Anti- IgE Mediated Histamine Release from RPMCs --- p.67 === Chapter 3.4 --- Discussion --- p.69 === Chapter 4. --- Elucidating the Mechanism Underlying the Effects of Oestrogens on Rat Peritoneal Mast Cells --- p.110 === Chapter 4.1 --- Introduction --- p.110 === Chapter 4.2 --- Materials and Methods --- p.115 === Chapter 4.3 --- Results --- p.116 === Chapter 4.3.1 --- The Involvement of Membrane Oestrogen Receptors --- p.117 === Chapter 4.3.1.1 --- Effects of Membrane Impermeable Oestrogen on Anti-IgE mediated Histamine Release from RPMCs --- p.117 === Chapter 4.3.2 --- Effects of Oestrogen Receptor Antagonists on the Modulating Actions of Oestrogen Receptor Agonists and SERMs on RPMC of Male Rats --- p.118 === Chapter 4.3.2.1 --- Effects on Oestrogen Receptor Agonists and SERMs Induced Low Level Histamine Release from RPMCs --- p.118 === Chapter 4.3.2.2 --- Effects on the Inhibitory Actions on Anti-IgE Mediated Histamine Release from RPMCs --- p.118 === Chapter 4.3.3 --- Effects of Transcription and Translation Blockers on the Inhibition Effects of Oestrogen Receptor Agonists and SERMs on RPMC of Male Rats --- p.122 === Chapter 4.3.4 --- Identification of Oestrogen Receptor Subtypes on RPMCs --- p.123 === Chapter 4.3.4.1 --- Molecular Expression of Oestrogen Receptor Subtypes on RPMCs --- p.123 === Chapter 4.3.5 --- Effects of Oestrogenic Agents on activation of RPMCs by Non- Immunological Stimuli --- p.123 === Chapter 4.3.5.1 --- Effects of Oestrogen on Compound48/80 Mediated Histamine Release from RPMCs --- p.123 === Chapter 4.3.5.2 --- Effects of Selective Oestrogen Receptor Modulators on Compound 48/80 Mediated Histamine Release from RPMCs --- p.124 === Chapter 4.3.5.3 --- Effects of Membrane Impermeable Oestrogen on Compound 48/80 Mediated Histamine Release from RPMCs --- p.125 === Chapter 4.3.5.4 --- Effects of Oestrogen on Substance P Mediated Histamine Release from RPMCs --- p.125 === Chapter 4.3.5.5 --- Effects of Selective Oestrogen Receptor Modulators on Substance P Mediated Histamine Release from RPMCs --- p.126 === Chapter 4.3.5.6 --- Effects of Membrane Impermeable Oestrogen on Substance P Mediated Histamine Release from RPMCs === Chapter 4.4 --- Discussion --- p.128 === References --- p.194