Expressed sequence tag (EST) analysis of black seabream (Acanthopagrus schlegeli) pituitary and seasonal analysis of gene expression in black seabream pituitary.

• Other Authors: Kong, Pui Shan.
• Format: Others
• Language: English; Chinese
• Published: 2005
• Subjects: Acanthopagrus > Genetics; Somatotropin; Pituitary gland
• Online Access: http://library.cuhk.edu.hk/record=b5892529; http://repository.lib.cuhk.edu.hk/en/item/cuhk-325243

Kong Pui Shan. === Thesis submitted in: November 2004. === Thesis (M.Phil.)--Chinese University of Hong Kong, 2005. === Includes bibliographical references (leaves 164-185). === Abstracts in English and Chinese. === Table of Contents --- p.i === List of Figures and Tables --- p.iii === List of Abbreviations --- p.viii === Chapter Chapter 1: --- Introduction --- p.1 === Chapter 1.1 --- Endocrine system and the pituitary gland in teleosts --- p.1 === Chapter 1.2 --- The biology of black seabream (Acanthopagrus schlegeli) --- p.4 === Chapter 1.3 --- Seasonal variation of gene expression in black seabream pituitary --- p.7 === Chapter 1.4 --- Expressed sequence tag (EST) analysis --- p.9 === Chapter 1.5 --- Basic Local Alignment Search Tool (BLAST) --- p.11 === Chapter 1.6 --- Aim of this study --- p.14 === Chapter Chapter 2: --- Materials and Methods --- p.15 === Chapter 2.1 --- Preliminary EST analysis of black seabream pituitary cDNA libraries --- p.15 === Chapter 2.2 --- Larger scale EST analysis of black seabream pituitary cDNA libraries --- p.19 === Chapter 2.3 --- "Northern blot analysis of gene expression levels of growth hormone (GH), prolactin (PRL), somatolactin (SL) and P-actin in adult female black seabream pituitaries" --- p.23 === Chapter 2.4 --- Real time-PCR analysis of seasonal expression of growth hormone family genes in black seabream pituitary --- p.25 === Chapter 2.5 --- Dot blot (miniarray) analysis of seasonal expression of different genes expressed in black seabream pituitary --- p.28 === Chapter Chapter 3: --- Preliminary EST Analysis of Black Seabream Pituitary cDNA Libraries --- p.30 === Chapter 3.1 --- Results and discussion --- p.30 === Chapter 3.1.1 --- EST analysis of black seabream pituitary cDNA libraries --- p.30 === Chapter 3.1.2 --- Full-length sequencing of different hormone genes of black seabream --- p.47 === Chapter Chapter 4: --- Larger Scale EST Analysis of Black Seabream Pituitary cDNA Libraries --- p.64 === Chapter 4.1 --- Results and discussion --- p.64 === Chapter 4.1.1 --- EST analysis of black seabream pituitary cDNA libraries --- p.64 === Chapter 4.1.2 --- Genes first time found in fish --- p.91 === Chapter Chapter 5: --- Analysis of Black Seabream Pituitary Gene Expressions in Pituitary Collected from Different Months --- p.103 === Chapter 5.1 --- Introduction --- p.103 === Chapter 5.2 --- Northern blot analysis of seasonal variation of growth hormone family genes --- p.105 === Chapter 5.3 --- Seasonal analysis of growth hormone family genes by real time-PCR --- p.110 === Chapter 5.4 --- Sexual maturity of black seabream throughout the reproductive cycle --- p.118 === Chapter 5.5 --- Seasonal analysis of black seabream pituitary genes by dot blot study --- p.119 === Chapter 5.6 --- Conclusion --- p.153 === Chapter Chapter 6: --- General Discussion --- p.154 === References --- p.164