Synthetic peptide studies on spike glycoprotein and 3C-like protease of the severe acute respiratory syndrome (SARS) coronavirus: perspective for SARS vaccine and drug development.

• Other Authors: Choy, Wai Yan.
• Format: Others
• Language: English; Chinese
• Published: 2005
• Subjects: Coronaviruses; Peptides > Therapeutic use; SARS (Disease) > Treatment; Drug development; SARS Virus; Peptides > therapeutic use; Severe Acute Respiratory Syndrome > drug therapy
• Online Access: http://library.cuhk.edu.hk/record=b5892338; http://repository.lib.cuhk.edu.hk/en/item/cuhk-325117

Choy Wai Yan. === Thesis (M.Phil.)--Chinese University of Hong Kong, 2005. === Includes bibliographical references (leaves 98-122). === Abstracts in English and Chinese. === Thesis committee --- p.i === Statement --- p.ii === Abstract --- p.iii === Acknowledgements --- p.vi === General abbreviations --- p.viii === Abbreviations of chemicals --- p.x === Table of contents --- p.xi === List of figures --- p.xv === List of tables --- p.xviii === Chapter 1 --- Introduction --- p.1 === Chapter 1.1 --- Severe acute respiratory syndrome (SARS) - An overview --- p.1 === Chapter 1.1.1 --- Epidemiology of SARS --- p.1 === Chapter 1.1.2 --- Clinical presentation of SARS --- p.2 === Chapter 1.1.3 --- Diagnostic tests of SARS --- p.5 === Chapter 1.1.4 --- Treatment of SARS --- p.7 === Chapter 1.2 --- Severe acute respiratory syndrome coronavirus (SARS- CoV) --- p.8 === Chapter 1.2.1 --- The etiological agent of SARS --- p.8 === Chapter 1.2.2 --- The coronaviruses --- p.9 === Chapter 1.2.3 --- Genome of SARS-CoV --- p.11 === Chapter 1.3 --- Spike (S) glycoprotein of SARS-CoV --- p.14 === Chapter 1.3.1 --- Functions of SARS-CoV S glycoprotein --- p.15 === Chapter 1.3.2 --- Receptors for S glycoprotein of SARS-CoV --- p.17 === Chapter 1.4 --- 3C-like protease (3CLPro) of SARS-CoV --- p.20 === Chapter 1.4.1 --- Extensive proteolytic processing of SARS-CoV replicase polyproteins --- p.20 === Chapter 1.4.2 --- SARS-CoV 3CLPro --- p.21 === Chapter 1.4.3 --- Substrate specificity of SARS-CoV 3CLPro --- p.22 === Chapter 1.5 --- Combating SARS - Vaccine and drug development --- p.24 === Chapter 1.5.1 --- Vaccine development against SARS --- p.24 === Chapter 1.5.2 --- Drug development against SARS --- p.25 === Chapter 1.6 --- Project objectives of this thesis --- p.27 === Chapter 1.6.1 --- Synthetic Peptide Studies on SARS-CoV S glycoprotein --- p.27 === Chapter 1.6.2 --- Synthetic Peptide Studies on SARS-CoV 3CLPro --- p.28 === Chapter 2 --- Materials and Methods --- p.30 === Chapter 2.1 --- Synthetic peptide studies on SARS-CoV S glycoprotein --- p.30 === Chapter 2.1.1 --- Bioinformatics analyses of SARS-CoV S gly- coprotein --- p.30 === Chapter 2.1.2 --- Peptide design and molecular modeling --- p.32 === Chapter 2.1.3 --- Solid phase peptide synthesis (SPPS) --- p.33 === Chapter 2.1.4 --- Peptide conjugation --- p.35 === Chapter 2.1.5 --- Immunization in rabbits and monkeys --- p.36 === Chapter 2.1.6 --- ELISA analysis --- p.37 === Chapter 2.1.7 --- Immunofluorescent confocal microscopy --- p.39 === Chapter 2.2 --- Synthetic peptide studies on SARS-CoV 3CLpro --- p.40 === Chapter 2.2.1 --- Protein expression and purification --- p.40 === Chapter 2.2.2 --- Solid phase peptide synthesis (SPPS) --- p.41 === Chapter 2.2.3 --- Peptide cleavage assay --- p.44 === Chapter 2.2.4 --- Molecular docking --- p.46 === Chapter 3 --- Results --- p.48 === Chapter 3.1 --- Synthetic peptide studies on SARS-CoV S glycoprotein --- p.48 === Chapter 3.1.1 --- General features and structural analyses of the S glycoprotein --- p.48 === Chapter 3.1.2 --- Peptides design and synthesis --- p.53 === Chapter 3.1.3 --- ELISA analysis and immunofluorescent con- focal microscopy --- p.55 === Chapter 3.2 --- Synthetic peptide studies on SARS-CoV 3CLpro --- p.62 === Chapter 3.2.1 --- Substrate specificity of SARS-CoV 3CLPro . . --- p.62 === Chapter 3.2.2 --- Molecular docking of SARS-CoV 3CLPro and peptide substrates --- p.74 === Chapter 4 --- Discussion --- p.78 === Chapter 4.1 --- Synthetic peptide studies on SARS-CoV S glycoprotein --- p.78 === Chapter 4.1.1 --- Synthetic peptides elicited SARS-CoV specific antibodies --- p.78 === Chapter 4.1.2 --- Factors affecting the specificity and antigenic- ity of synthetic peptides --- p.80 === Chapter 4.1.3 --- Next step towards vaccine development --- p.83 === Chapter 4.1.4 --- A synthetic peptide-based approach --- p.84 === Chapter 4.2 --- Synthetic peptide studies on SARS-CoV 3CLpro --- p.86 === Chapter 4.2.1 --- A comprehensive overview of the substrate specificity of SARS-CoV 3CLpro --- p.87 === Chapter 4.2.2 --- Sequence comparison between SARS-CoV 3CLpro cleavage sites --- p.90 === Chapter 4.2.3 --- A rapid and high throughput approach to screen protease substrate specificity --- p.94 === Bibliography --- p.98