Random coil phosphorus chemical shift of deoxyribonucleic acids.

• Other Authors: Ho, Cheuk-nang.
• Format: Others
• Language: English; Chinese
• Published: 2004
• Subjects: Phosphorus; DNA
• Online Access: http://library.cuhk.edu.hk/record=b5896196; http://repository.lib.cuhk.edu.hk/en/item/cuhk-324917

Ho Cheuk-nang. === Thesis (M.Phil.)--Chinese University of Hong Kong, 2004. === Includes bibliographical references (leaves 89-94). === Abstracts in English and Chinese. === Acknowledge --- p.ii === Table of Contents --- p.iii === Lists of Tables --- p.vi === Lists of Figures --- p.vii === Abstract (English Version) --- p.x === Abstract (Chinese Version) --- p.xii === Chapter 1. --- Literature Survey of Phosphorus Chemical Shift Studies of DNA --- p.1 === Chapter 1.1 --- Introduction --- p.1 === Chapter 1.2 --- General Review of DNA Structures --- p.2 === Chapter 1.2.1 --- "Nomenclature, Symbols and Numbering Scheme" --- p.2 === Chapter 1.2.2 --- Conformations of DNA --- p.7 === Chapter 1.2.3 --- Random Coil State --- p.8 === Chapter 1.3 --- Phosphorus Chemical Shift Studies of DNA --- p.9 === Chapter 1.4 --- Purpose of This Work --- p.10 === Chapter 2. --- Methodology for Studying the Sequence Effect on Random Coil Phosphorus Chemical Shift --- p.12 === Chapter 2.1 --- Introduction --- p.12 === Chapter 2.2 --- Determination of Sequence Effect --- p.12 === Chapter 2.3 --- Design of Sequence --- p.13 === Chapter 2.4 --- Sample Preparation --- p.14 === Chapter 2.5 --- Measurement of Phosphorus Chemical Shift --- p.16 === Chapter 2.5.1 --- Proton Resonance Assignments --- p.16 === Chapter 2.5.2 --- Phosphorus Resonance Assignments --- p.20 === Chapter 2.6 --- Determination of Sugar Conformation --- p.23 === Chapter 2.7 --- Determination of Backbone Conformation --- p.25 === Chapter 3. --- Results and Discussion of Random Coil Phosphorus Chemical Shift of DNA --- p.28 === Chapter 3.1 --- Introduction --- p.28 === Chapter 3.2 --- Resonance Assignments --- p.28 === Chapter 3.3.1 --- Proton Resonance Assignments --- p.31 === Chapter 3.3.2 --- Phosphorus Resonance Assignments --- p.31 === Chapter 3.3 --- Verification of Random Coil State --- p.31 === Chapter 3.2.1 --- Variable Temperature Proton Chemical Shift --- p.31 === Chapter 3.2.2 --- Sugar Conformation --- p.32 === Chapter 3.2.3 --- Backbone Conformation --- p.33 === Chapter 3.4 --- Random Coil Phosphorus Chemical Shift of DNA --- p.33 === Chapter 3.4.1 --- Temperature Effect --- p.33 === Chapter 3.4.2 --- Neighbor Effect --- p.36 === Chapter 3.5 --- Random Coil Phosphorus Chemical Shift Prediction --- p.42 === Chapter 3.5.1 --- Dimer Model Prediction Protocol --- p.42 === Chapter 3.5.2 --- Trimer Model Prediction Protocol --- p.45 === Chapter 4. --- Conclusions and Future Work --- p.48 === Appendix I H6/H8-H1' regions of 2D NOESY spectra of SS1-SS16 --- p.49 === "Appendix II 'H and 31P resonance assignments and 3Jhi1,h2,3Jh1，h2´ح and 3Jh3，p coupling constants of SS1-SS16" --- p.57 === Appendix III H3' regions 1H-31P HSQC spectra of SS1-SS16 --- p.65 === Appendix IV H1，-H2，/H2´حregions of DQF-COSY spectra of SS1- SS16 --- p.73 === Appendix V H3' regions of 1H-31P selective heteronuclear COSY spectra of SS1-SS16 --- p.81 === References --- p.89