The antiproliferative activity of hakmeitau bean (Vigna sinensis) extract.

• Other Authors: Lau, Wing-Sze.
• Format: Others
• Language: English; Chinese
• Published: 2004
• Subjects: Legumes > Seeds > Composition; Legumes > Physiological effect; Antioxidants
• Online Access: http://library.cuhk.edu.hk/record=b5892192; http://repository.lib.cuhk.edu.hk/en/item/cuhk-324606

Lau Wing-Sze. === Thesis (M.Phil.)--Chinese University of Hong Kong, 2004. === Includes bibliographical references (leaves 131-149). === Abstracts in English and Chinese. === Acknowledgements --- p.i === Abstract --- p.ii === Abstract (Chinese version) --- p.iv === Table of Contents --- p.vi === List of Tables --- p.x === List of Figures --- p.xii === List of Abbreviations --- p.xiv === Chapter Chapter One: --- An overview of Vigna sinensis seeds === Chapter 1.1 --- Introduction --- p.1 === Chapter 1.2 --- Food and functional food --- p.2 === Chapter 1.3 --- Edible legumes as an important food --- p.4 === Chapter 1.4 --- Nutritional an extra-nutritional values of V. sinensis seeds --- p.5 === Chapter Chapter Two: --- Purification of phenolic antioxidants from V. sinensis seeds === Chapter 2.1 --- Introduction --- p.11 === Chapter 2.1.1 --- Reactive oxygen species and antioxidants --- p.12 === Chapter 2.1.2 --- Phenolic flavonoids --- p.15 === Chapter 2.2 --- Materials and Methods === Chapter 2.2.1 --- Chemicals and reagents --- p.24 === Chapter 2.2.2 --- Plant material --- p.25 === Chapter 2.2.3 --- Optimization and extraction of V. sinensis seeds constituents --- p.25 === Chapter 2.2.4 --- Chromatographic separation of phenolic components --- p.26 === Chapter 2.2.5 --- Determination of phenolic contents --- p.27 === Chapter 2.2.6 --- Determination of free radical scavenging ability using trolox equivalent antioxidant capacity (TEAC) assay --- p.28 === Chapter 2.2.7 --- Statistical analysis --- p.29 === Chapter 2.3 --- Results and Discussion === Chapter 2.3.1 --- Optimization on the extraction of V. sinensis seeds --- p.30 === Chapter 2.3.2 --- Extraction and fractionation of V. sinensis seeds constituents --- p.31 === Chapter 2.3.3 --- Yield of various V sinensis seed fractions --- p.31 === Chapter 2.3.4 --- Phenolic contents in various V. sinensis seed fractions --- p.32 === Chapter 2.3.5 --- Free radical scavenging abilities of various V sinensis seed fractions --- p.33 === Chapter Chapter Three: --- Effect of V. sinensis seed extract on high fat and cholesterol - feeding mice === Chapter 3.1 --- Introduction --- p.41 === Chapter 3.1.1 --- Cholesterol in bloodstream circulation --- p.42 === Chapter 3.1.2 --- "Relationship between LDL oxidation, atherosclerosis and coronary heart disease" --- p.43 === Chapter 3.1.3 --- Diet supplements with beneficial effects on preventing coronary heart disease --- p.44 === Chapter 3.2 --- Materials and Methods --- p.47 === Chapter 3.2.1 --- Chemicals and reagents --- p.47 === Chapter 3.2.2 --- Preparation of diets --- p.48 === Chapter 3.2.3 --- Animals --- p.48 === Chapter 3.2.4 --- Feeding experiments --- p.49 === Chapter 3.2.5 --- Post-feeding analysis --- p.50 === Chapter 3.2.5.1 --- Caecal content and health indices --- p.50 === Chapter 3.2.5.2 --- Serum triglycerides --- p.51 === Chapter 3.2.5.3 --- Serum total cholesterol --- p.52 === Chapter 3.2.5.4 --- High-density lipoprotein (HDL) cholesterol --- p.53 === Chapter 3.2.5.5 --- Low-density lipoprotein (LDL) cholesterol --- p.54 === Chapter 3.2.5.6 --- Hepatic lipid and cholesterol --- p.55 === Chapter 3.2.6 --- Statistical analysis --- p.55 === Chapter 3.3 --- Results and Discussion --- p.56 === Chapter 3.3.1 --- Food intakes and body weights of animals --- p.56 === Chapter 3.3.2 --- Caecal contents and health indices --- p.56 === Chapter 3.3.3 --- Effects of V sinensis seed extract on serum and hepatic levels of triglycerides and cholesterol --- p.57 === Chapter Chapter Four: --- Antiproliferative activities of V. sinensis seed extracts === Chapter 4.1 --- Introduction --- p.66 === Chapter 4.1.1 --- Cancer and antioxidant --- p.67 === Chapter 4.1.2 --- Dietary cancer prevention agents --- p.68 === Chapter 4.2 --- Materials and Methods --- p.71 === Chapter 4.2.1 --- Chemicals and reagents --- p.71 === Chapter 4.2.2 --- Cell lines --- p.71 === Chapter 4.2.3 --- Maintenance of cell lines --- p.72 === Chapter 4.2.4 --- Antiproliferation assays --- p.73 === Chapter 4.2.4.1 --- MTT assay --- p.73 === Chapter 4.2.4.2 --- BrdU assay --- p.73 === Chapter 4.2.5 --- Cytotoxic activity determined by lactate dehydrogenase (LDH) assay --- p.77 === Chapter 4.2.6 --- Time-course assay --- p.79 === Chapter 4.2.7 --- Determination of IC50 --- p.79 === Chapter 4.2.8 --- Statistical analysis --- p.79 === Chapter 4.3 --- Results and Discussion --- p.80 === Chapter 4.3.1 --- Antiproliferative activities of V. sinensis seed extracts on HepG2 cells --- p.80 === Chapter 4.3.2 --- Cytotoxic activities of V. sinensis seed extracts on HepG2 cells --- p.82 === Chapter 4.3.3 --- Antiproliferative activities of phenolic fraction on MCF-7cells --- p.83 === Chapter 4.3.4 --- Cytotoxic activity of phenolic fraction on MCF-7 cells --- p.84 === Chapter 4.3.5 --- Time-course study of antiproliferative activities of phenolic fraction on cancer cells --- p.85 === Chapter 4.3.6 --- Effect of phenolic fraction on normal cells --- p.86 === Chapter Chapter Five: --- Antioxidant and antiproliferative activities of selected content flavonoids from V. sinensis seeds === Chapter 5.1 --- Introduction --- p.93 === Chapter 5.1.1 --- Cell cycle progression and regulation --- p.94 === Chapter 5.1.2 --- Bioavailability of plant flavonoids --- p.96 === Chapter 5.1.3 --- Characterization of flavonoids in V. sinensis seed --- p.98 === Chapter 5.2 --- Materials and Methods --- p.102 === Chapter 5.2.1 --- Chemicals and reagents --- p.102 === Chapter 5.2.2 --- Determination of free radical scavenging ability of identified flavonoids from V sinensis seeds using trolox equivalent antioxidant capacity (TEAC) assay --- p.103 === Chapter 5.2.3 --- Antiproliferation assays --- p.104 === Chapter 5.2.4 --- Cytotoxicity assay --- p.104 === Chapter 5.2.5 --- Time-course assay --- p.104 === Chapter 5.2.6 --- Determination of cell cycle distribution by flow cytometry --- p.105 === Chapter 5.2.7 --- Statistical analysis --- p.106 === Chapter 5.3 --- Results and Discussion --- p.107 === Chapter 5.3.1 --- Free radical scavenging abilities of identified flavonoids from V sinensis seeds --- p.107 === Chapter 5.3.2 --- Antiproliferative activities of selected flavonoids on cancer cells --- p.109 === Chapter 5.3.3 --- Cytotoxic activities of selected flavonoids on cancer cells --- p.111 === Chapter 5.3.4 --- Time -course study of antiproliferative activities on cancer cells --- p.112 === Chapter 5.3.5 --- Cytotoxic activities of selected flavonoids on normal cells --- p.114 === Chapter 5.3.6 --- Determination of the effects of cyanidin on cancer cells by analyzing cell cycle pattern --- p.115 === Chapter Chapter Six: --- Conclusion --- p.128 === References --- p.131