Characteristics of cells under different tumor microenvironmental conditions.

• Other Authors: Ng, Mei Yu.
• Format: Others
• Language: English; Chinese
• Published: 2002
• Subjects: Neoplasms > genetics; Neoplasms > physiopathology; Epidermal Growth Factor
• Online Access: http://library.cuhk.edu.hk/record=b5891023; http://repository.lib.cuhk.edu.hk/en/item/cuhk-323848

by Ng Mei Yu. === Thesis (M.Phil.)--Chinese University of Hong Kong, 2002. === Includes bibliographical references (leaves 173-183). === Abstracts in English and Chinese. === Acknowledgements --- p.2 === Abbreviations --- p.3 === Abstracts --- p.4 === List of figures and tables --- p.7 === Contents Page No === General Introduction --- p.13 === Chapter CHAPTER ONE --- Biological Characterization of A431 Cells & SiHa Cells Under Different Microenvironments --- p.16 === Chapter 1.1 --- Introduction --- p.17 === Chapter 1.1.1 --- Microenvironment Surrounding Tumor Cells --- p.18 === Chapter 1.1.2 --- Hypoxic Environment --- p.20 === Chapter 1.1.2.1 --- The Hypoxic Chamber --- p.20 === Chapter 1.1.3 --- Reoxygenation --- p.22 === Chapter 1.1.4 --- Acidic Environment --- p.23 === Chapter 1.1.5 --- Glucose Depletion --- p.24 === Chapter 1.1.6 --- Irradiation --- p.25 === Chapter 1.2 --- Objectives --- p.26 === Chapter 1.3 --- Materials and Methods --- p.27 === Chapter 1.3.1 --- Materials --- p.27 === Chapter 1.3.2 --- Methods --- p.29 === Chapter 1.3.2.1 --- Cell Lines --- p.29 === Chapter 1.3.2.2 --- The Working Procedure for the Hypoxic Chamber --- p.30 === Chapter 1.3.2.3 --- "Aerobic, Hypoxic and Reoxygenated Conditions" --- p.33 === Chapter 1.3.2.4 --- Acidic Condition --- p.35 === Chapter 1.3.2.5 --- Glucose Depleted Condition --- p.36 === Chapter 1.3.2.6 --- Gamma-Irradiation --- p.37 === Chapter 1.3.2.7 --- Analysis of the Growth Pattern by MTT Assay and Cell Counting --- p.38 === Chapter 1.3.2.8 --- Cell Cycle Analysis --- p.39 === Chapter 1.3.2.9 --- Western Blot Analysis --- p.40 === Chapter 1.3.2.10 --- DNA Fragmentation Analysis --- p.42 === Chapter 1.4 --- Results --- p.43 === Chapter 1.4.1 --- Cell Proliferation Profile by MTT Assay --- p.43 === Chapter 1.4.1.1 --- Proliferation of cells under hypoxia --- p.43 === Chapter 1.4.1.2 --- Proliferation of cells under acidic pH environments --- p.49 === Chapter 1.4.1.3 --- Proliferation of cells under glucose depleted environment --- p.52 === Chapter 1.4.2 --- Distribution of cell cycles under different micro environments --- p.54 === Chapter 1.4.3 --- General Protein Expression Pattern by Western Blot Analysis --- p.57 === Chapter 1.4.4 --- Detection of Apoptosis by DNA Fragmentation Assay --- p.59 === Chapter 1.5 --- Discussion --- p.58 === Chapter CHAPTER TWO --- REACTION KINETICS OF A431 CELLS AND SiHa CELLS INDUCED BY EGF --- p.71 === Chapter 2.1 --- Introduction --- p.72 === Chapter 2.1.1 --- Structure of EGF and EGFR --- p.74 === Chapter 2.1.2 --- EGF Signaling Pathway --- p.76 === Chapter 2.2 --- Objectives --- p.79 === Chapter 2.3 --- Materials and Methods --- p.80 === Chapter 2.3.1 --- Materials --- p.80 === Chapter 2.3.2 --- Methods --- p.82 === Chapter 2.3.2.1 --- Cell Lines --- p.82 === Chapter 2.3.2.2 --- EGF Sensitivity Assay --- p.83 === Chapter 2.3.2.3 --- Combination Effect of Hypoxia and EGF --- p.83 === Chapter 2.3.2.4 --- Early Kinetics Analysis by Low EGF Concentration Treatment --- p.84 === Chapter 2.3.2.5 --- Late Kinetics Analysis by High EGF Concentration Treatment --- p.85 === Chapter 2.4 --- Results --- p.86 === Chapter 2.4.1 --- Sensitivity of A431 cells and SiHa cells to EGF by MTT Assay --- p.86 === Chapter 2.4.2 --- Early/Late Kinetics of EGF induced protein tyrosine phosphorylation Pattern --- p.90 === Chapter 2.4.3 --- Raf protein expression --- p.96 === Chapter 2.4.4 --- EGFR expression level --- p.100 === Chapter 2.5 --- Discussions --- p.104 === Chapter CHAPTER THREE --- IDENTIFICATION OF DIFFERENTIALLY EXPRESSED GENES IN A431 CELLS BY DIFFERENTIAL DISPLAY UNDER DIFFERENT TUMOR MICROENVIRONMENTS --- p.107 === Chapter 3.1 --- Introduction --- p.108 === Chapter 3.2 --- Materials and Methods --- p.112 === Chapter 3.2.1 --- Materials --- p.112 === Chapter 3.2.2 --- Methods --- p.114 === Chapter 3.2.2.1 --- Spheroid Cells --- p.114 === Chapter 3.2.2.2 --- Identification of Differentally Expressed Genes by RT-PCR --- p.117 === Chapter 3.2.2.3 --- Ligation and Cloning of Differentially Expressed cDNA --- p.120 === Chapter 3.2.2.4 --- Screening and Sequencing of the cDNA Inserts --- p.121 === Chapter 3.2.2.5 --- Northern Blot Analysis --- p.123 === Chapter 3.3 --- Results --- p.124 === Chapter 3.4 --- Discussions --- p.161 === GENERAL CONCLUSION --- p.165 === REFERENCES --- p.167