Expression regulation of endometrial ion channels by steroid hormones.
Tsang Lai-Ling Angel. === Thesis (M.Phil.)--Chinese University of Hong Kong, 2001. === Includes bibliographical references (leaves 136-145). === Abstracts in English and Chinese. === Abstract --- p.i === 論文撮要 --- p.iv === Acknowledgment --- p.vi === Table of Content --- p.vii === List of Publica...
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| Format: | Others |
| Language: | English Chinese |
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2001
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| Online Access: | http://library.cuhk.edu.hk/record=b5890909 http://repository.lib.cuhk.edu.hk/en/item/cuhk-323647 |
| Summary: | Tsang Lai-Ling Angel. === Thesis (M.Phil.)--Chinese University of Hong Kong, 2001. === Includes bibliographical references (leaves 136-145). === Abstracts in English and Chinese. === Abstract --- p.i === 論文撮要 --- p.iv === Acknowledgment --- p.vi === Table of Content --- p.vii === List of Publications --- p.xii === List of Figures --- p.xiv === List of Tables --- p.xvii === Abbreviations --- p.xviii === Chapter Chapter1 --- Introduction --- p.1 === Chapter 1.1 --- The Human Uterus Vs Rat Uterus --- p.1 === Chapter 1.1.1 --- Myometrium --- p.1 === Chapter 1.1.2 --- Endometrium --- p.1 === Chapter 1.2 --- The Human Endometrium Vs Rat Endometrium --- p.2 === Chapter 1.2.1 --- The structure of Human Endometrium --- p.2 === Chapter 1.2.2 --- Cyclic Changes in the Endometrium --- p.4 === Chapter 1.2.3 --- Physiological Roles of the Endometrium --- p.7 === Chapter 1.2.4 --- Uterine Fluid Volume and its Composition --- p.7 === Chapter 1.2.4.1 --- Regulation of Uterine Fluid Volume and Composition --- p.7 === Chapter 1.2.4.2 --- Role of Endometrial Epithelium in the Regulation of Uterine Fluid Volume --- p.9 === Chapter 1.3 --- Epithelial Ion Channels --- p.9 === Chapter 1.3.1 --- Epithelial CI- Channels in Secretory Epithelia --- p.11 === Chapter 1.3.1.1 --- Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) --- p.13 === Chapter 1.3.2 --- Epithelial Na+ Channel (ENaC) in Absorbing Epithelia --- p.18 === Chapter 1.3.3 --- ENaC and CFTR in Endometrial Epithelia --- p.26 === Chapter 1.4 --- Hormonal Regulation of the Endometrial Epithelium --- p.29 === Chapter 1.4.1 --- Estrogen and Progesterone --- p.29 === Chapter 1.4.2 --- Aldosterone --- p.32 === Chapter 1.5 --- Aim of Study --- p.35 === Chapter Chapter2 --- Materials and Methods --- p.38 === Chapter 2.1 --- Materials --- p.38 === Chapter 2.1.1 --- Culture Medium and Enzymes --- p.38 === Chapter 2.1.2 --- Drugs --- p.38 === Chapter 2.1.3 --- Molecular Biology --- p.39 === Chapter 2.1.4 --- Experimental Tissues and Animals --- p.39 === Chapter 2.2 --- Preparations --- p.39 === Chapter 2.2.1 --- Pervious Support for Cell Growth --- p.39 === Chapter 2.2.2 --- Growth Medium --- p.40 === Chapter 2.2.3 --- Culture of Mouse Endometrium Epithelial Cells --- p.43 === Chapter 2.2.4 --- Solutions for the Short-Circuit Current Measurement --- p.44 === Chapter 2.2.5 --- Electrodes for the Short-Circuit Current Measurement --- p.44 === Chapter 2.2.6 --- Solutions for Molecular Biology Experiment --- p.44 === Chapter 2.2.6.1 --- Diethyl Pyrocarbonate (DEPC)-treated Water --- p.44 === Chapter 2.2.6.2 --- lx TAE (DNA gel electrophoresis and its running buffer) --- p.45 === Chapter 2.2.6.3 --- 5x MOPS (RNA gel electrophoresis and its running buffer) --- p.45 === Chapter 2.2.6.4 --- Formaldehyde Gel-loading Buffer --- p.45 === Chapter 2.3 --- Protocols --- p.46 === Chapter 2.3.1 --- Effect of Ovarian Hormones and Aldosterone on CFTR and ENaC Expression --- p.45 === Chapter 2.3.2 --- Possible Interaction between CFTR and ENaC upon Hormones Stimulation --- p.47 === Chapter 2.4 --- Methods of Measurement --- p.48 === Chapter 2.4.1 --- The Short-Circuit Current Technique --- p.48 === Chapter 2.4.1.1 --- The Short-Circuit Current Setup --- p.48 === Chapter 2.4.1.2 --- Experimental Procedures --- p.52 === Chapter 2.4.1.3 --- Data Analysis --- p.55 === Chapter 2.4.2 --- Reverse Transcription - Polymerase Chain Reaction (RT-PCR) --- p.55 === Chapter 2.4.2.1 --- RNA Isolation --- p.55 === Chapter 2.4.2.2 --- RNA Gel Electrophoresis --- p.56 === Chapter 2.4.2.3 --- Reverse Transcription (RT) --- p.57 === Chapter 2.4.2.4 --- Primer used for the Polymerase Chain Reaction (PCR) --- p.58 === Chapter 2.4.2.5 --- General Procedure of PCR and Competitive RT-PCR --- p.59 === Chapter 2.4.2.6 --- DNA Gel Electrophoresis --- p.61 === Chapter 2.4.3 --- Capillary Electrophoresis - Laser Induced Fluorescence (CE-LIF) --- p.62 === Chapter 2.4.3.1 --- Capillary Tube --- p.54 === Chapter 2.4.3.2 --- Detection System --- p.65 === Chapter 2.4.3.3 --- Experimental Procedures --- p.65 === Chapter 2.4.3.4 --- Data Analysis --- p.66 === Chapter 2.4.4 --- Statistical Analysis === Chapter Chapter3 --- Results --- p.68 === Chapter 3.1 --- Influence of Ovarian Hormones on Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) and Epithelial Na+ Channel (ENaC) Expression in Mouse Endometrial Epithelium --- p.68 === Chapter 3.2 --- Culture Condition on Expression and Function of Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) in Mouse Endometrial Epithelial Cells --- p.92 === Chapter 3.3 --- Expression Regulation of Endometrial Epithelial Na+ Channel (ENaC) Subunits and Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) by Na+ Diet During the Estrus Cycle in Mice --- p.98 === Chapter 3.4 --- Enhanced Epithelial Na+ Channel (ENaC) Activity in Mouse Endometrial Epithelium by Upregulation of γ-ENaC Subunit --- p.114 === Chapter Chapter4 --- General Discussion --- p.127 === Appendix --- p.132 === Chapter A. --- RNA Isolation --- p.132 === Chapter B. --- Reverse Transcription (RT) --- p.133 === Chapter C. --- Polymerase Chain Reaction (PCR) --- p.134 === Chapter D. --- Sequences and Conditions of All Primers --- p.135 === References --- p.136 |
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