Microsatellite instability and its significance in cervical and endometrial cancers.

• Other Authors: Ip, Toi Yan.
• Format: Others
• Language: English; Chinese
• Published: 1999
• Subjects: Uterine Cervical Neoplasms; Endometrial Neoplasms; Microsatellite Repeats
• Online Access: http://library.cuhk.edu.hk/record=b5889973; http://repository.lib.cuhk.edu.hk/en/item/cuhk-322868

Ip Toi Yan. === Thesis (M.Phil.)--Chinese University of Hong Kong, 1999. === Includes bibliographical references (leaves 81-105). === Abstracts in English and Chinese. === CONTENTS --- p.i-iii === ACKNOWLEDGEMENT --- p.iv === ABSTRACT --- p.v-vi === Chapter Chapter One --- INTRODUCTION --- p.1-2 === Chapter Chapter Two --- LITERATURE REVIEW --- p.3-37 === Chapter 2.1 --- Epidemiology and Etiology of Cervical and Endometrial Cancers --- p.3-4 === Chapter 2.1.1 --- Epidemiology and Etiology of Cervical cancer --- p.4 === Chapter 2.1.1.1 --- Incidence and Mortality --- p.4-6 === Chapter 2.1.1.2 --- Etiology --- p.6-8 === Chapter 2.1.2 --- Epidemiology and Etiology of Endometrial Cancer --- p.9 === Chapter 2.1.2.1 --- Incidence and Mortality --- p.9-11 === Chapter 2.1.2.2 --- Rick Factors --- p.11-14 === Chapter 2.2 --- Pathology of Cervical and Endometrial Cancers --- p.14 === Chapter 2.2.1 --- Pathology of Cervical Cancer --- p.14-15 === Chapter 2.2.1.1 --- Macroscopic Appearance --- p.15 === Chapter 2.2.1.2 --- Histology --- p.15-18 === Chapter 2.2.2 --- Staging of Cervical Cancer --- p.19-21 === Chapter 2.2.3 --- Pathology of Endometrial Cancer --- p.21 === Chapter 2.2.3.1 --- Macroscopic Appearance --- p.22 === Chapter 2.2.3.2 --- Histology --- p.22-24 === Chapter 2.2.4 --- Staging of Endometrial Cancer --- p.24-25 === Chapter 2.2 --- Introduction to Microsatellite Instability (MI) --- p.25 === Chapter 2.3.1 --- DNA structure --- p.25-27 === Chapter 2.3.2 --- Microsatellite --- p.27-28 === Chapter 2.3.3 --- Mismatch Repair (MMR) --- p.28-29 === Chapter 2.3.4 --- Microsatellite Instability (MI) --- p.30-33 === Chapter 2.3.5 --- Microsatellite Instability in various cancers --- p.33-37 === Chapter Chapter Three --- MATERIALS AND METHODS --- p.38-50 === Chapter 3.1 --- Materials --- p.38 === Chapter 3.1.1 --- Patients and Specimens --- p.38-39 === Chapter 3.1.2 --- Chemicals and Reagents --- p.39 === Chapter 3.1.2.1 --- Chemicals --- p.39-40 === Chapter 3.1.2.2 --- Solution --- p.40-41 === Chapter 3.1.2.3 --- Microsatellite Markers --- p.42 === Chapter 3.1.3 --- Major Equipment --- p.43 === Chapter 3.2 --- Methodology --- p.43 === Chapter 3.2.1 --- DNA Extraction --- p.43-45 === Chapter 3.2.2 --- DNA Amplification --- p.45 === Chapter 3.2.2.1 --- End-labeling of Primer --- p.45 === Chapter 3.2.2.2 --- Polymerase Chain Reaction (PCR) --- p.46 === Chapter 3.2.3 --- Electrophoresis of PCR Products and Autoradiography --- p.46-49 === Chapter 3.2.4 --- Determination Of Microsatellite Instability (MI) --- p.49 === Chapter 3.3 --- Statistical Analyses --- p.50 === Chapter Chapter Four --- Result --- p.51-66 === Chapter 4.1 --- Microsatellite Instability in Cervical Cancer --- p.51 === Chapter 4.1.1 --- Prevalence of MI in Cervical Cancer --- p.51 -54 === Chapter 4.1.2 --- MI and Age in Cervical Cancer --- p.55 === Chapter 4.1.3 --- MI and Histological Type in Cervical Cancer --- p.55-56 === Chapter 4.1.4 --- MI and Histologic Grades in Cervical Cancer --- p.56-57 === Chapter 4.1.5 --- MI and Clinical stage in Cervical Cancer --- p.57-58 === Chapter 4.1.6 --- MI and Clinical Status in Cervical Cancer --- p.58-59 === Chapter 4.2 --- Microsatellite Instability in Endometrial Cancer --- p.59 === Chapter 4.2.1 --- Prevalence of MI in Endometrial Cancer --- p.59-62 === Chapter 4.2.2 --- MI and Age Groups in Endometrial Cancer --- p.63 === Chapter 4.2.3 --- MI and Histological Type in Endometrial Cancer --- p.63-64 === Chapter 4.2.4 --- MI and Histologic Grades in Endometrial Cancer --- p.64-65 === Chapter 4.2.5 --- MI and Clinical stage of Endometrial Cancer --- p.65 === Chapter 4.2.6 --- MI and Clinical Status in Endometrial Cancer --- p.66 === Chapter Chapter Five --- Discussion --- p.67-77 === Chapter 5.1 --- MI detection --- p.67-71 === Chapter 5.2 --- MI of Cervical Cancer --- p.71 -74 === Chapter 5.3 --- MI of Endometrial Cancer --- p.74-77 === Chapter Chapter Six --- Conclusions --- p.78-80 === Reference --- p.81-112 === Appendix --- p.113-114