Inhibition of leukemic apoptosis by antisense oligonucleotide.

• Other Authors: Lai, Wing Hong Kevin.
• Format: Others
• Language: English
• Published: Chinese University of Hong Kong 1995
• Subjects: Apoptosis; Leukemia > Ultrastructure; Ouabain > Therapeutic use; Oligonucleotides > Therapeutic use; Proto-Oncogene proteins > Physiology
• Online Access: http://library.cuhk.edu.hk/record=b5888522; http://repository.lib.cuhk.edu.hk/en/item/cuhk-320716

by Lai Wing Hong Kevin. === Thesis (M.Phil.)--Chinese University of Hong Kong, 1995. === Includes bibliographical references (leaves 63-74). === Acknowledgments --- p.i === Abbreviations --- p.ii === Abstract --- p.1 === Chapter Chapter 1 --- General Introduction --- p.3 === Chapter 1.1 --- Advantages of Antisense Oligonucleotides Inhibition --- p.4 === Chapter 1.2 --- The Uses of Antisense Oligonucleotide in Leukemic Therapy --- p.5 === Chapter 1.3 --- Oncogenes in the Pathogenesis of Leukemia --- p.6 === Chapter 1.4 --- Apoptosis and Apoptosis-Related Genes --- p.9 === Chapter 1.5 --- Protooncogene bcl-2 --- p.10 === Chapter 1.6 --- Bcl-2 Homologues --- p.11 === Chapter 1.7 --- Regulation of Apoptosis by Other Genes --- p.13 === Chapter 1.8 --- Promyelocytic Leukemia HL-60 Cell Line --- p.15 === Chapter 1.9 --- Aim of Project --- p.16 === Chapter Chapter 2 --- Chemical Synthesis of DNA Oligonucleotides === Chapter 2.1 --- Introduction --- p.17 === Chapter 2.2 --- Materials and Methods --- p.20 === Chapter 2.3 --- Results --- p.24 === Chapter 2.4 --- Discussion --- p.26 === Chapter Chapter 3 --- The Apoptotic Effects of TPA and Ouabain on the Promyelocytic Leukemic HL-60 cell line === Chapter 3.1 --- Introduction --- p.30 === Chapter 3.2 --- Materials and methods --- p.33 === Chapter 3.3 --- Results --- p.40 === Chapter 3.4 --- Discussion --- p.44 === Chapter Chapter 4 --- Effect of Antisense Oligonucleotides on TPA-Induced Apoptosisin Leukemic HL-60 cells === Chapter 4.1 --- Introduction --- p.48 === Chapter 4.2 --- Materials and Methods --- p.49 === Chapter 4.3 --- Results --- p.52 === Chapter 4.4 --- Discussion --- p.54 === Chapter Chapter 5 --- General Discussion --- p.57 === References --- p.63