Molecular epidemiology of salmonella typhi in Hong Kong.

• Other Authors: Lo, Norman Wai-sing.
• Format: Others
• Language: English
• Published: Chinese University of Hong Kong 1994
• Subjects: Salmonella typhimurium; Molecular Epidemiology
• Online Access: http://library.cuhk.edu.hk/record=b5888620; http://repository.lib.cuhk.edu.hk/en/item/cuhk-320701

by Norman Wai-sing Lo. === Thesis (M.Phil.)--Chinese University of Hong Kong, 1994. === Includes bibliographical references (leaves 145-160). === Chapter Chapter 1 --- Introduction --- p.1 === Chapter A. --- Classification of Salmonella --- p.3 === Chapter B. --- Enteric fever --- p.4 === Chapter C. --- Chloramphenicol and multiple resistance in S. Typhi --- p.7 === Chapter D. --- Resistance plasmids in the typhoid bacillus --- p.11 === Chapter E. --- In vivo acquisition of resistance plasmids by S. Typhi --- p.18 === Chapter F. --- Worldwide distribution of typhoid fever --- p.23 === Chapter G. --- Epidemiology of typhoid fever in Hong Kong --- p.27 === Chapter H. --- Principles of methods used in the epidemiological typing of S. Typhi --- p.29 === Chapter 1. --- Phage typing --- p.29 === Chapter 2. --- Antibiotics sensitivity pattern --- p.32 === Chapter 3. --- Plasmid analyses --- p.32 === Chapter a. --- Genetic methods --- p.33 === Chapter i. --- Phenotypic expression --- p.33 === Chapter ii. --- Transferability of plasmids --- p.33 === Chapter iii. --- Incompatibility --- p.34 === Chapter b. --- Molecular methods --- p.34 === Chapter i. --- Estimation of molecular size by agarose gel electrophoresis --- p.34 === Chapter ii. --- Plasmid fingerprinting --- p.35 === Chapter iii. --- Localization of resistance genes by DNA-DNA hybridization --- p.35 === Chapter 4. --- Ribotyping --- p.36 === Chapter 5. --- Total DNA fingerprinting --- p.37 === Chapter 6. --- Chromosomal insertion sequence IS200 profile analysis --- p.38 === Chapter 7. --- Others --- p.39 === Chapter a. --- Multilocus enzyme electrophoresis --- p.39 === Chapter b. --- Phenotype of lipopolysaccharide (LPS) --- p.39 === Chapter c. --- Envelope protein profiles and immunoblotting --- p.40 === Chapter I. --- Epidemiological typing of S. Typhi --- p.40 === Chapter J. --- Objectives --- p.46 === Chapter Chapter 2 --- Materials and Methods --- p.48 === Chapter A. --- Materials --- p.48 === Chapter I. --- Bacterial strains --- p.48 === Chapter II. --- Materials --- p.51 === Chapter B. --- Methods --- p.56 === Chapter I. --- Sensitivity testing --- p.56 === Chapter II. --- Characterization of plasmids --- p.58 === Chapter 1. --- Genetic studies --- p.58 === Chapter a. --- Transferability of resistance plasmids --- p.58 === Chapter b. --- Mobilization of resistances --- p.59 === Chapter c. --- Incompatibility grouping --- p.59 === Chapter 2. --- Molecular studies --- p.60 === Chapter a. --- Plasmid profile analysis --- p.60 === Chapter i. --- Plasmid extraction --- p.60 === Chapter ii. --- Agarose gel electrophoresis --- p.61 === Chapter b. --- Molecular characterization of plasmid --- p.61 === Chapter i. --- Extraction of purified plasmid DNA --- p.62 === Chapter ii. --- Restriction endonuclease digestion of plasmid DNA --- p.63 === Chapter III. --- Characterization β-lactamases --- p.63 === Chapter 1. --- Extraction of β-lactamases --- p.63 === Chapter 2. --- Determination of isoelectric points (pIs) --- p.64 === Chapter IV. --- Localization of resistance genes --- p.64 === Chapter 1. --- TEM-1 and TEM-2 genes --- p.65 === Chapter a. --- Oligonucleotide probes --- p.65 === Chapter b. --- Total DNA preparation --- p.66 === Chapter c. --- DNA-DNA-hybridization --- p.66 === Chapter i. --- Transfer of DNA to membrane filters --- p.66 === Chapter ii. --- Labelling of oligonucleotide probes (TEM-1 and TEM-2) --- p.67 === Chapter iii. --- Hybridization --- p.68 === Chapter 2. --- Chloramphenicol- and tetracycline-resistance genes --- p.69 === Chapter a. --- Preparation of probes --- p.69 === Chapter b. --- Hybridization --- p.73 === Chapter i. --- Transfer of DNA to membrane filters --- p.73 === Chapter ii. --- Labelling of chloramphenicol- and tetracycline- resistance probes --- p.73 === Chapter V. --- Epidemiological typing --- p.75 === Chapter 1. --- Ribotyping --- p.75 === Chapter 2. --- DNA fingerprinting --- p.77 === Chapter C. --- Plan for achieving objectives --- p.77 === Chapter Chapter 3 --- Results --- p.79 === Chapter A. --- Antimicrobial susceptibilities --- p.79 === Chapter B. --- Characterization of resistance plasmids --- p.83 === Chapter C. --- β-lactamases produced by ampicillin-resistant S. Typhi --- p.85 === Chapter D. --- Localization of resistance genes --- p.85 === Chapter E. --- Plasmid profile analysis --- p.87 === Chapter F. --- Plasmid fingerprinting --- p.90 === Chapter G. --- Ribotyping --- p.93 === Chapter H. --- Chromosomal DNA fingerprinting --- p.107 === Chapter I. --- "Correlation of PstI, ClaI and KpnI ribotypes and NarI, EcoRV and MluI chromosomal types" --- p.124 === Chapter J. --- Correlation of PstI ribotypes and NarI chromosomal types --- p.126 === Chapter K. --- Epidemiology of S. Typhi in Hong Kong --- p.129 === Chapter Chapter 4 --- Discussion --- p.131 === Chapter A. --- Antimicrobial susceptibilities --- p.131 === Chapter B. --- Characterization of resistances --- p.132 === Chapter C. --- Plasmid profile analysis --- p.135 === Chapter D. --- Epidemiological analysis of S. Typhi --- p.136 === Chapter E. --- Area for future research --- p.143 === References --- p.145 === Appendix --- p.161 === Chapter A. --- Buffer and Stock solutions --- p.161 === Chapter B. --- Epidemiological information of S. Typhi isolates --- p.167