Quantitation of iron in the liver, pancreas and heart of hospital patients in Hong Kong.

• Other Authors: Kwong, Yim-kam.
• Format: Others
• Language: English
• Published: Chinese University of Hong Kong 1993
• Subjects: Iron > analysis; Liver > anatomy & histology; Patients; Patients > Hong Kong
• Online Access: http://library.cuhk.edu.hk/record=b5887704; http://repository.lib.cuhk.edu.hk/en/item/cuhk-319251

by Yim-kam Kwong. === Thesis (M.Phil.)--Chinese University of Hong Kong, 1993. === Includes bibliographical references (leaves 119-133). === ACKNOWLEDGEMENT --- p.vii === LIST OF TABLES --- p.viii === LIST OF FIGURES --- p.x === ABSTRACT --- p.1 === SECTION === Chapter 1. --- INTRODUCTION --- p.3 === Chapter 2. --- LITERATURE REVIEW --- p.6 === Chapter 3. --- MATERIALS AND METHODS --- p.39 === Chapter 4. --- RESULTS --- p.61 === Chapter 5. --- DISCUSSION --- p.103 === Chapter 6. --- CONCLUSION --- p.116 === REFERENCES --- p.119 === APPENDIX --- p.134 === Chapter SECTION 1 --- INTRODUCTION --- p.3 === Chapter SECTION 2 --- LITERATURE REVIEW --- p.6 === Chapter 2.1 --- IRON --- p.6 === Chapter 2.1.1 --- CHEMISTRY --- p.6 === Chapter 2.1.2 --- METABOLISM --- p.6 === Chapter 2.1.2.1 --- Homeostasis --- p.6 === Chapter 2.1.2.2 --- Absorption --- p.8 === Chapter 2.1.2.3 --- Transportation - Role of transferrin in iron transport --- p.9 === Chapter 2.1.2.4 --- Storage --- p.10 === Ferritin --- p.11 === Haemosiderin --- p.13 === Chapter 2.2 --- IRON OVERLOAD --- p.14 === Chapter 2.2.1 --- AETIOLOGY --- p.14 === Chapter 2.2.2 --- PREVALENCE --- p.15 === Chapter 2.2.3 --- MECHANISM --- p.16 === Chapter 2.2.4 --- PATHOLOGY OF IRON OVERLOAD --- p.17 === Chapter 2.2.4.1 --- Increased absorption of iron from the diet --- p.18 === Chapter 2.2.4.2 --- Parenteral administration of excess iron --- p.21 === Chapter 2.2.4.3 --- Increased iron absorption combined with transfusional overload --- p.22 === Chapter 2.2.4.4 --- Miscellaneous conditions --- p.23 === Chapter 2.2.5 --- CLINICAL PRESENTATION --- p.24 === Chapter 2.2.6 --- EFFECT OF IRON OVERLOAD --- p.25 === Chapter 2.2.6.1 --- Role of iron in lipid peroxidation --- p.25 === Chapter 2.2.6.2 --- Iron and neoplasia --- p.26 === Chapter 2.3 --- ASSESSMENT OF IRON OVERLOAD --- p.26 === Chapter 2.3.1 --- NON-SERUM PARAMETER --- p.26 === Chapter 2.3.1.1 --- Localization of stored iron --- p.27 === Chapter 2.3.1.2 --- Morphometric assessment of hepatic iron in liver biopsy --- p.30 === Chapter 2.3.1.3 --- Hepatic iron concentration --- p.31 === Chapter 2.3.1.4 --- Atomic absorption spectrophotometry --- p.32 === Chapter 2.3.1.5 --- Hepatic imaging studies --- p.33 === Chapter 2.3.2 --- SERUM PARAMETERS --- p.34 === Chapter 2.3.2.1 --- Serum ferritin measurement --- p.34 === Chapter 2.3.2.2 --- Serum iron --- p.36 === Chapter 2.3.2.4 --- Transferrin saturation --- p.37 === Chapter SECTION 3 --- MATERIALS AND METHOD --- p.39 === Chapter 3.1 --- SUBJECTS --- p.39 === Chapter 3.1.1 --- SOURCE OF TISSUE SAMPLES AND CASE SELECTION --- p.39 === Chapter 3.1.1.1 --- The controls --- p.39 === Chapter 3.1.1.2 --- The transfusion group --- p.39 === Chapter 3.1.1.3 --- The non-transfusion group --- p.40 === Chapter 3.1.1.4 --- The total group --- p.40 === Chapter 3.2 --- METHODS --- p.40 === Chapter 3.2.1. --- HISTOLOGICAL METHOD --- p.44 === Chapter 3.2.1.1 --- Haematoxylin and Eosin Stain --- p.47 === Chapter 3.2.1.2 --- Perls' Prussian Blue Method --- p.49 === Chapter 3.2.1.3 --- The Rowe's Method of Iron Deposition --- p.47 === Chapter 3.2.1.4 --- Method 1 --- p.48 === Chapter 3.2.1.5 --- Method2 Estimation and grouping of % area --- p.49 === Chapter 3.2.1.6 --- "Comparison of Rowe's method, and the two histological iron grading methods" --- p.54 === Chapter 3.2.2 --- CHEMICAL MEASUREMENT --- p.55 === Chapter 3.2.2.1 --- Sectioning of paraffin liver blocks for chemical measurement --- p.55 === Chapter 3.2.2.2 --- Paraffin removal --- p.56 === Chapter SECTION 4 --- RESULTS --- p.61 === Chapter 4.1 --- HISTOLOGICAL ASSESSMENT --- p.61 === Chapter 4.1.1 --- HISTOLOGICAL STUDY --- p.61 === Chapter 4.1.2 --- SEX DISTRIBUTION --- p.65 === Chapter 4.1.3 --- AGE DISTRIBUTION --- p.65 === Chapter 4.2 --- CHEMICAL MEASUREMENT --- p.81 === Chapter 4.2.1 --- EVALUATION OF ANALYTICAL PRECISION --- p.84 === Chapter 4.2.2 --- RESULT OF CHEMICAL MEASUREMENTS --- p.81 === Chapter 4.2.3 --- ASSOCIATED CONDITIONS IN PATIENTS WITH LIVER TISSUE IRON > 50 μMOL/G --- p.86 === Chapter 4.3 --- CORRELATION OF HISTOLOGICAL ASSESSMENT WITH CHEMICAL MEASUREMENT --- p.88 === Chapter 4.3.1 --- CORRELATION OF HISTOLOGICAL ASSESSMENT WITH CHEMICAL MEASUREMENT BY METHOD 1 --- p.88 === Chapter 4.3.2 --- CORRELATION OF ASSESSMENT WITH CHEMICAL MEASUREMENT BY METHOD 2 --- p.89 === Chapter 4.3.2.1 --- Percentage area --- p.95 === Chapter 4.3.2.2 --- Score --- p.96 === Chapter 4.4 --- PANCREATIC AND MYOCARDIAC HAEMOSIDEROSIS --- p.100 === Chapter 4.4.1 --- METHOD 2 --- p.100 === Chapter SECTION 5 --- DISCUSSIONS --- p.103 === Chapter SECTION 6 --- CONCLUSIONS --- p.116 === REFERENCES --- p.119 === APPENDIX --- p.134