Determination of Fe, Cu and Zn in human plasma by energy dispersive X-ray fluorescence spectrometry.

• Other Authors: Chan, Wing-yee.
• Format: Others
• Language: English
• Published: Chinese University of Hong Kong 1993
• Subjects: X-ray spectroscopy; Blood proteins > Analysis
• Online Access: http://library.cuhk.edu.hk/record=b5887819; http://repository.lib.cuhk.edu.hk/en/item/cuhk-319226

by Chan Wing-yee. === Thesis (M.Phil.)--Chinese University of Hong Kong, 1993. === Includes bibliographical references (leaves 93-95). === ACKNOWLEDGEMENT --- p.i === ABSTRACT --- p.ii === Chapter CHAPTER 1 --- INTRODUCTION --- p.1 === Chapter 1.1 --- General Introduction --- p.1 === Chapter 1.2 --- "Clinical Significance of Fe, Cu and Zn" --- p.2 === Chapter 1.3 --- Alternative Methods of Analysis --- p.5 === Chapter 1.4 --- Principles of Energy Dispersive X-ray Fluorescence Spectrometry --- p.11 === Chapter 1.5 --- Research Plan --- p.20 === Chapter CHAPTER 2 --- EXPERIMENTAL --- p.22 === Chapter 2.1 --- Energy Dispersive X-ray Fluorescence Analysis --- p.22 === Chapter 2.1.1 --- Apparatus --- p.22 === Chapter 2.1.2 --- Reagents --- p.25 === Chapter 2.1.3 --- Procedure --- p.28 === Chapter 2.2 --- Atomic Absorption Spectrometric Analysis --- p.32 === Chapter 2.2.1 --- Apparatus --- p.32 === Chapter 2.2.2 --- Reagents --- p.32 === Chapter 2.2.3 --- Procedure --- p.34 === Chapter CHAPTER 3 --- RESULTS AND DISCUSSION --- p.38 === Chapter 3.1 --- Optimisation of Excitation Conditions --- p.38 === Chapter 3.1.1 --- Effect of Filter --- p.38 === Chapter 3.1.2 --- Effect of Tube Voltage --- p.43 === Chapter 3.1.3 --- Effect of Tube Current --- p.44 === Chapter 3.2 --- Optimisation of Preconcentration Procedure --- p.46 === Chapter 3.2.1 --- Effect of Sample Area and Collimator Size --- p.46 === Chapter 3.2.2 --- Effect of pH --- p.51 === Chapter 3.2.3 --- Effect of Ligand Concentration --- p.54 === Chapter 3.2.4 --- Effect of Mixing Time --- p.57 === Chapter 3.2.5 --- Effect of Standing Time --- p.59 === Chapter 3.2.6 --- Study of Sample Homogeneity --- p.61 === Chapter 3.3 --- Optimisation for Deproteination --- p.63 === Chapter 3.3.1 --- Effect of Different Protein Precipitants --- p.63 === Chapter 3.3.2 --- Effect of Trichloroacetic Acid Concentration --- p.65 === Chapter 3.3.3 --- Effect of Hydrochloric Acid Concentration --- p.67 === Chapter 3.3.4 --- Effect of Temperature --- p.69 === Chapter 3.3.5 --- Effect of Incubation Time --- p.71 === Chapter 3.4 --- Study of Blanks --- p.74 === Chapter 3.5 --- Construction of Calibration Curves --- p.77 === Chapter 3.6 --- Determination of Detection Limit and Sensitivity --- p.84 === Chapter 3.7 --- Accuracy and Reproducibility Tests --- p.86 === Chapter 3.8 --- Parallel Check --- p.89 === Chapter CHAPTER 4 --- CONCLUSION --- p.92 === REFERENCES --- p.93