Genetical and cytological studies on variations of volvariella volvacea.

• Other Authors: Li, Shuxian
• Format: Others
• Language: English
• Published: Chinese University of Hong Kong 1991
• Subjects: Edible mushrooms
• Online Access: http://library.cuhk.edu.hk/record=b5886950; http://repository.lib.cuhk.edu.hk/en/item/cuhk-318766

by Li Shuxian. === Thesis (Ph.D.)--Chinese University of Hong Kong, 1991. === Bibliography: leaves 169-192. === ACKNOWLEDGEMENTS --- p.ii === ABSTRACT --- p.iii === LIST OF TABLES --- p.viii === LIST OF FIGURES --- p.x === ABBREVIATIONS --- p.xiv === Chapter CHAPTER ONE: --- GENERAL INTRODUCTION --- p.1 === Chapter CHAPTER TWO: --- LITERATURE REVIEW === Chapter 2.1. --- Introduction --- p.6 === Chapter 2.2. --- Sexuality --- p.6 === Chapter 2.2.1. --- Heterothallism --- p.9 === Chapter 2.2.2. --- Homothallism --- p.11 === Chapter 2.2.3. --- Sexuality of Volvariella volvacea --- p.14 === Chapter 2.3. --- "Induction and selection of genetic ., markers in higher fungi" --- p.18 === Chapter 2.3.1. --- Physical mutagenesis --- p.19 === Chapter 2.3.2. --- Chemical mutagenesis --- p.22 === Chapter 2.3.3. --- Biological mutagenesis --- p.24 === Chapter 2.4. --- Nuclear behaviour in edible fungi --- p.27 === Chapter 2.4.1. --- Somatic division --- p.27 === Chapter 2.4.2. --- Meiotic events --- p.28 === Chapter 2.4.3. --- Electrophoretic karyotype --- p.31 === Chapter CHAPTER THREE : --- VARIATIONS IN MONOSPOROUS ISOLATES OF VOLVARIELLA VOLVACEA === Chapter 3.1. --- Introduction --- p.32 === Chapter 3.2. --- Materials and methods --- p.33 === Chapter 3.2.1. --- Strains and culture media --- p.33 === Chapter 3.2.2. --- Monosporous isolation --- p.34 === Chapter 3.2.3. --- Linear and radial growth --- p.34 === Chapter 3.2.4. --- Fertility test --- p.35 === Chapter 3.2.5. --- Determination of extracellular cellulase activity --- p.33 === Chapter 3.3. --- Results --- p.41 === Chapter 3.3.1. --- Variations in colonial morphology --- p.41 === Chapter 3.3.2. --- Variations in linear growth rate --- p.44 === Chapter 3.3.3. --- Variations in fertility --- p.46 === Chapter 3.3.4. --- A comparison of extracellular cellulase activity between fertile and sterile monosporous isolates --- p.52 === Chapter 3.4. --- Discussion --- p.61 === Chapter 3.4.1. --- Study on the colonial morphology of monosporous isolates --- p.61 === Chapter 3.4.2. --- Study on the growth rate of monosporous isolates --- p.62 === Chapter 3.4.3. --- Study on the extracellular cellulase activity --- p.63 === Chapter 3.4.4. --- Study on the fertility of monosporous isolates --- p.64 === Chapter 3.5. --- Summary --- p.66 === Chapter CHAPTER FOUR: --- GENETICAL STUDIES ON VARIATIONS OF V. VOLVACEA === Chapter 4.1. --- Introduction --- p.67 === Chapter 4.2. --- Materials and methods --- p.68 === Chapter 4.2.1. --- Strains and cultural condition --- p.68 === Chapter 4.2.2. --- Chemical compounds --- p.70 === Chapter 4.2.3. --- Determination of sensitivities to growth inhibitor and chemical --- p.72 === Chapter 4.2.4. --- UV mutagenesis --- p.72 === Chapter 4.2.5. --- Chemical mutagenesis --- p.73 === Chapter 4.2.6. --- Enrichment methods for auxotrophs --- p.74 === Chapter 4.2.7. --- Isolation and establishment of resistance markers --- p.74 === Chapter 4.2.8. --- Isolation and characterization of auxotrophic mutants --- p.75 === Chapter 4.2.9. --- Fruiting and marker segregation test --- p.75 === Chapter 4.3. --- Results --- p.75 === Chapter 4.3.1. --- Effects of UV irradiation on mycelium and spore germination rate --- p.76 === Chapter 4.3.2. --- Median lethal concentrations (LC50) of chemical mutagens --- p.79 === Chapter 4.3.3. --- Sensitivity of various strains to growth inhibitors and tested compounds --- p.82 === Chapter 4.3.4. --- Identification and characterization of resistant mutants --- p.94 === Chapter 4.3.5. --- Identification and characterization of auxotrophic mutants --- p.115 === Chapter 4.3.6. --- Analysis of genetic markers in progenies of mutants --- p.116 === Chapter 4.4. --- Discussion --- p.118 === Chapter 4.4.1. --- Significance of mutant induction in V. volvacea --- p.119 === Chapter 4.4.2. --- Methods for mutant induction --- p.120 === Chapter 4.4.3. --- Resistant mutants of V. volvacea --- p.121 === Chapter 4.4.4. --- Auxotrophic mutants of V. volvacea --- p.124 === Chapter 4.4.5. --- Study on the sexuality pattern of V.volvacea by analyzing mutant progeny --- p.125 === Chapter 4.5. --- Summary --- p.128 === Chapter CHAPTER FIVE: --- CYTOLOGICAL STUDIES ON VARIATIONS OF V. VOLVACEA === Chapter 5.1. --- Introduction --- p.129 === Chapter 5.2. --- Materials and methods --- p.130 === Chapter 5.2.1. --- Strains --- p.130 === Chapter 5.2.2. --- Feulgen staining method --- p.130 === Chapter 5.2.3. --- Fluorescent staining of nuclei with DAPI --- p.131 === Chapter 5.2.4. --- Microscopic autoradiography --- p.131 === Chapter 5.2.5. --- Scanning electron microscopic examination --- p.132 === Chapter 5.2.6. --- DNA measurement --- p.132 === Chapter 5.3. --- Results --- p.133 === Chapter 5.3.1. --- Number of nuclei in V. volvacea --- p.133 === Chapter 5.3.2. --- Comparison of cytological differences between self-fertile and self-sterile monosporous isolates --- p.143 === Chapter 5.3.3. --- Spore patterns on basidia --- p.145 === Chapter 5.3.4. --- Nuclear DNA content of V.volvacea --- p.148 === Chapter 5.4. --- Discussion --- p.154 === Chapter 5.4.1. --- Staining method --- p.154 === Chapter 5.4.2. --- Analysis of the possible sources of variation in V. volvacea --- p.155 === Chapter 5.5. --- Summary --- p.162 === Chapter CHAPTER SIX: --- GENERAL SUMMARY AND CONCLUSION --- p.164 === REFERENCES: --- p.169