Three-dimensional bone and cancer cell model in roller tube and chicken chorioallantoic membrane

• Main Author: Ratanasirintrawoot, Adisa
• Other Authors: Salih, Erdjan
• Language: en_US
• Published: 2021
• Subjects: Dentistry
• Online Access: https://hdl.handle.net/2144/42810

BACKGROUND: Oral squamous cell carcinoma is one of the most commonly found cancer and often metastasizes to bone. Bone-cancer models were developed throughout the years in order to replicate true bone-cancer relationships with dynamic 3D ex-vivo model utilizing calvarial bone displaying the closest relationship to natural bone cancer environment. AIM : to create a closer ex-vivo environment to study the relationship between bone and OSCC, utilizing the dynamic three-dimensional ex-vivo model in combination with the abundant vasculature from CAM. MATERIALS AND METHODS: Mice calvarial bone were extracted and incubated in roller tube then transferred to chicken chorioallantoic membrane. Experimental samples were introduced to oral squamous cell carcinoma prior to incubation. Samples were separated into bone resorption phenomenon and bone formation phenomenon by including ascorbic acid into the environment. Samples were collected at 7 and 14 days and was processed under histological analysis and fluorescent assay. RESULTS: Histological analysis showed that in both bone resorption and bone formation phenomenon, OSCC stimulate bone resorption process and causes bone necrosis with large mass of inflammatory cells. There is a significant reduction in OSCC in samples that were incubated in roller tube and chicken CAM compared to samples that were incubated in roller tube only. CONCLUSION: OSCC has the natural characteristic of osteoclasts similar to those findings for breast cancer cells. It also induced bone necrosis and data suggests OSCC may metastasize through the chick’s circulation to other embryo sites. Future investigations need to be conducted due to small sample size and high standard deviation.