Biophysical changes in cell membranes during myogenesis 'in vitro'

• Main Author: Santini, Maria T.
• Language: en_US
• Published: Boston University 2019
• Online Access: https://hdl.handle.net/2144/38100

Thesis (Ph.D.)--Boston University === PLEASE NOTE: Boston University Libraries did not receive an Authorization To Manage form for this thesis or dissertation. It is therefore not openly accessible, though it may be available by request. If you are the author or principal advisor of this work and would like to request open access for it, please contact us at [e-mail]. Thank you. === The differentiation of chick embryo myoblasts in vitro was studied using electron paramagnetic resonance (EPR) and dielectric relaxation. Cell membranes of myoblasts in rotation culture for 72 hours were examined with three nitroxystearate (NSA) spin labels which insert at increasingly greater depths into the lipid bilayer (5-NSA < 12-NSA < 16-NSA). Changes in membrane order during myogenic differentiation in vitro were seen. The spin label which inserts near the bilayer surface (5-NSA) showed two peaks of membrane order: one at 15 hours (associated with gyratory aggregation and recovery of the myoblasts from dissociation) and one at 38 hours (associated with prostaglandin binding and increased cell ... [TRUNCATED] === 2031-01-01