Isolation of antibodies to outer membrane proteins from nontypable Haemophilus influenza

Isolation of antibodies to outer membrane proteins from nontypable Haemophilus influenza

Thesis (M.A.)--Boston University === PLEASE NOTE: Boston University Libraries did not receive an Authorization To Manage form for this thesis or dissertation. It is therefore not openly accessible, though it may be available by request. If you are the author or principal advisor of this work and wou...

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Main Author: Hamburger, Jonathan
Language:en_US
Published: Boston University 2019
Subjects:
Antibodies
Influenza
Microbiology
Immunization
Online Access:https://hdl.handle.net/2144/37159
id ndltd-bu.edu-oai-open.bu.edu-2144-37159
record_format oai_dc
spelling ndltd-bu.edu-oai-open.bu.edu-2144-371592019-12-07T03:03:03Z Isolation of antibodies to outer membrane proteins from nontypable Haemophilus influenza Hamburger, Jonathan Antibodies Influenza Microbiology Immunization Thesis (M.A.)--Boston University PLEASE NOTE: Boston University Libraries did not receive an Authorization To Manage form for this thesis or dissertation. It is therefore not openly accessible, though it may be available by request. If you are the author or principal advisor of this work and would like to request open access for it, please contact us at open-help@bu.edu. Thank you. Outer membrane proteins from unencapsulated(nontypable) Haemophilus influenza were prepared by incubation with EDTA and shearing followed by molecular sieve chromatography in a lipooligosaccharide (LOS) disaggregating deoxycholate buffer. Outer membranes proteins collected were determined to be free of LOS by polyacrylamide gel electrophoresis and then covalently attached to cyanogen bromide activated Sepharose. Antibodies to outer membrane proteins were then isolated by affinity chromatography and characterized by enzyme-linked immunosorbant assay. These antibodies will prove useful for passive immunization experiments to establish the role of outer membrane proteins in host protection from infection with nontypable Haemophilus influenza. 2031-01-01 2019-08-19T12:07:15Z 1988 1988 Thesis/Dissertation b18377713 https://hdl.handle.net/2144/37159 11719015086632 99187678640001161 en_US Boston University
collection NDLTD
language en_US
sources NDLTD
topic Antibodies
Influenza
Microbiology
Immunization
spellingShingle Antibodies
Influenza
Microbiology
Immunization
Hamburger, Jonathan
Isolation of antibodies to outer membrane proteins from nontypable Haemophilus influenza
description Thesis (M.A.)--Boston University === PLEASE NOTE: Boston University Libraries did not receive an Authorization To Manage form for this thesis or dissertation. It is therefore not openly accessible, though it may be available by request. If you are the author or principal advisor of this work and would like to request open access for it, please contact us at open-help@bu.edu. Thank you. === Outer membrane proteins from unencapsulated(nontypable) Haemophilus influenza were prepared by incubation with EDTA and shearing followed by molecular sieve chromatography in a lipooligosaccharide (LOS) disaggregating deoxycholate buffer. Outer membranes proteins collected were determined to be free of LOS by polyacrylamide gel electrophoresis and then covalently attached to cyanogen bromide activated Sepharose. Antibodies to outer membrane proteins were then isolated by affinity chromatography and characterized by enzyme-linked immunosorbant assay. These antibodies will prove useful for passive immunization experiments to establish the role of outer membrane proteins in host protection from infection with nontypable Haemophilus influenza. === 2031-01-01
author Hamburger, Jonathan
author_facet Hamburger, Jonathan
author_sort Hamburger, Jonathan
title Isolation of antibodies to outer membrane proteins from nontypable Haemophilus influenza
title_short Isolation of antibodies to outer membrane proteins from nontypable Haemophilus influenza
title_full Isolation of antibodies to outer membrane proteins from nontypable Haemophilus influenza
title_fullStr Isolation of antibodies to outer membrane proteins from nontypable Haemophilus influenza
title_full_unstemmed Isolation of antibodies to outer membrane proteins from nontypable Haemophilus influenza
title_sort isolation of antibodies to outer membrane proteins from nontypable haemophilus influenza
publisher Boston University
publishDate 2019
url https://hdl.handle.net/2144/37159
work_keys_str_mv AT hamburgerjonathan isolationofantibodiestooutermembraneproteinsfromnontypablehaemophilusinfluenza
_version_ 1719302102510993408

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